Measurement of MicroRNA: A Regulator of Gene Expression

Elena Flowers, Erika Sivarajan Froelicher, Bradley E. Aouizerat

Research output: Contribution to journalArticle

Abstract

MicroRNAs (miRs) are epigenetic regulators of messenger RNAs' (mRNA) expression of polypeptides. As such, miRs represent an intriguing mechanism by which gene-environment interactions are hypothesized to occur on the level of epigenetic control over gene expression. In addition to promising findings from in vitro studies indicating that miRs have the potential to function as therapeutic agents in modifying the course of pathophysiologic conditions, recent human studies revealed changes in miR expression patterns in response to behavioral interventions. The authors provide an overview of how miRs are preserved and isolated from other genetic material and describe commonly used methods for measuring miR in the research setting, including Northern blot, polymerase chain reaction, and microarray. The authors also introduce bioinformatic approaches to analysis of high-throughput miR expression and techniques used to create predictive models of miR-mRNA binding to describe possible physiologic pathways affected by specific miRs.

Original languageEnglish (US)
Pages (from-to)167-178
Number of pages12
JournalBiological Research for Nursing
Volume15
Issue number2
DOIs
StatePublished - 2013

Keywords

  • epigenetics
  • measurement
  • microRNA
  • microarray

ASJC Scopus subject areas

  • Research and Theory

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