Measurement of Prostaglandin E2 in Interstitial Fluid From the Dog Stomach After Feeding and Indomethacin

N. W. Bunnett, J. H. Walsh, H. T. Debas, G. L. Kauffman, E. M. Golanska

Research output: Contribution to journalArticle

Abstract

The purpose of the study was to develop a method for collecting interstitial fluid bathing the stomach tissues in which prostaglandins could be measured. Hollow dialysis fibers attached at the ends to Silastic tubes were surgically implanted into the submucosa of the gastric fundus and antrum of dogs. The Silastic tubes were exteriorized through the body wall. After full recovery from surgery, the fibers were filled with 5% bovine serum albumin in isotonic saline that was replaced at 5-min intervals. Prostaglandin E2 was measured in the dialysate by radioimmunoassay. In 6 dogs, feeding significantly stimulated the release of prostaglandin E2 into the fundic interstitial fluid from 5.3 ± 0.6 ng · ml-1 to 12.1 ± 1.6 ng · ml-1 (p < 0.01) but had no effect on antral levels. In 4 dogs, indomethacin (0.01, 0.1, 1.0, and 10.0 ng · kg-1, i.v.) caused a dose-dependent depression in prostaglandin E2 levels in interstitial fluid of the fundus and antrum. In 4 other dogs, indomethacin depressed the ex vivo generation of prostaglandin E2 in biopsy specimens of the fundus and antrum. These results validate the technique of interstitial fluid dialysis and suggest that it is a powerful method for examining the secretion of locally acting substances in the stomach of conscious animals.

Original languageEnglish (US)
Pages (from-to)1391-1398
Number of pages8
JournalGastroenterology
Volume85
Issue number6
DOIs
StatePublished - 1983

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

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