Measuring cellular ion transport by magnetoencephalography

Sudhir Kumar Sharma; Sauparnika Vijay; Sangram Gore; Timothy M. Dore; Ramesh Jagannathan

Measuring cellular ion transport by magnetoencephalography

Sudhir Kumar Sharma, Sauparnika Vijay, Sangram Gore, Timothy M. Dore, Ramesh Jagannathan

Research output: Other contribution

Abstract

The cellular-level process of ion transport is known to generate a magnetic field. A non-invasive magnetoencephalography (MEG) technique was used to measure the magnetic field emanating from HeLa, HEK293 and H9c2(2-1) rat cardiac cells. The addition of a non-lethal dose of ionomycin to HeLa and capsaicin to TRPV1-expressing HEK293 cells, respectively, resulted in a sudden change in the magnetic field signal consistent with Ca2+ influx, which was also observed by confocal fluorescence microscopy under the same conditions. In contrast, addition of capsaicin to TRPV1-expressing HEK293 cells containing an optimum amount of Ca2+ channel blockers, a TRPV1 antagonist (ruthenium red), resulted in no detectable magnetic or fluorescent signals. These signals confirmed that the measured MEG signals are due to cellular ion transport through the cell membrane. In general, there is evidence that ion channel/transporter activation and ionic flux are linked to cancer; Therefore, our work suggests that MEG could represent a non-invasive method for detecting cancer.

Original language	English (US)
Publisher	arXiv
State	Published - Mar 5 2020

Publication series

Name	arXiv

Keywords

Ca 2+ channel blockers
H9c2(2-1) rat cardiac cells
HEK293 cells
HeLa cells
Magnetic fields
Magnetoencephalography(MEG)

Fingerprint Dive into the research topics of 'Measuring cellular ion transport by magnetoencephalography'. Together they form a unique fingerprint.

Cite this

@misc{380f934a300c4c0c934af7f502e03c41,

title = "Measuring cellular ion transport by magnetoencephalography",

abstract = "The cellular-level process of ion transport is known to generate a magnetic field. A non-invasive magnetoencephalography (MEG) technique was used to measure the magnetic field emanating from HeLa, HEK293 and H9c2(2-1) rat cardiac cells. The addition of a non-lethal dose of ionomycin to HeLa and capsaicin to TRPV1-expressing HEK293 cells, respectively, resulted in a sudden change in the magnetic field signal consistent with Ca2+ influx, which was also observed by confocal fluorescence microscopy under the same conditions. In contrast, addition of capsaicin to TRPV1-expressing HEK293 cells containing an optimum amount of Ca2+ channel blockers, a TRPV1 antagonist (ruthenium red), resulted in no detectable magnetic or fluorescent signals. These signals confirmed that the measured MEG signals are due to cellular ion transport through the cell membrane. In general, there is evidence that ion channel/transporter activation and ionic flux are linked to cancer; Therefore, our work suggests that MEG could represent a non-invasive method for detecting cancer.",

keywords = "Ca 2+ channel blockers, H9c2(2-1) rat cardiac cells, HEK293 cells, HeLa cells, Magnetic fields, Magnetoencephalography(MEG)",

author = "Sharma, {Sudhir Kumar} and Sauparnika Vijay and Sangram Gore and Dore, {Timothy M.} and Ramesh Jagannathan",

year = "2020",

month = mar,

day = "5",

language = "English (US)",

series = "arXiv",

publisher = "arXiv",

type = "Other",

}

TY - GEN

T1 - Measuring cellular ion transport by magnetoencephalography

AU - Sharma, Sudhir Kumar

AU - Vijay, Sauparnika

AU - Gore, Sangram

AU - Dore, Timothy M.

AU - Jagannathan, Ramesh

PY - 2020/3/5

Y1 - 2020/3/5

N2 - The cellular-level process of ion transport is known to generate a magnetic field. A non-invasive magnetoencephalography (MEG) technique was used to measure the magnetic field emanating from HeLa, HEK293 and H9c2(2-1) rat cardiac cells. The addition of a non-lethal dose of ionomycin to HeLa and capsaicin to TRPV1-expressing HEK293 cells, respectively, resulted in a sudden change in the magnetic field signal consistent with Ca2+ influx, which was also observed by confocal fluorescence microscopy under the same conditions. In contrast, addition of capsaicin to TRPV1-expressing HEK293 cells containing an optimum amount of Ca2+ channel blockers, a TRPV1 antagonist (ruthenium red), resulted in no detectable magnetic or fluorescent signals. These signals confirmed that the measured MEG signals are due to cellular ion transport through the cell membrane. In general, there is evidence that ion channel/transporter activation and ionic flux are linked to cancer; Therefore, our work suggests that MEG could represent a non-invasive method for detecting cancer.

AB - The cellular-level process of ion transport is known to generate a magnetic field. A non-invasive magnetoencephalography (MEG) technique was used to measure the magnetic field emanating from HeLa, HEK293 and H9c2(2-1) rat cardiac cells. The addition of a non-lethal dose of ionomycin to HeLa and capsaicin to TRPV1-expressing HEK293 cells, respectively, resulted in a sudden change in the magnetic field signal consistent with Ca2+ influx, which was also observed by confocal fluorescence microscopy under the same conditions. In contrast, addition of capsaicin to TRPV1-expressing HEK293 cells containing an optimum amount of Ca2+ channel blockers, a TRPV1 antagonist (ruthenium red), resulted in no detectable magnetic or fluorescent signals. These signals confirmed that the measured MEG signals are due to cellular ion transport through the cell membrane. In general, there is evidence that ion channel/transporter activation and ionic flux are linked to cancer; Therefore, our work suggests that MEG could represent a non-invasive method for detecting cancer.

KW - Ca 2+ channel blockers

KW - H9c2(2-1) rat cardiac cells

KW - HEK293 cells

KW - HeLa cells

KW - Magnetic fields

KW - Magnetoencephalography(MEG)

UR - https://www.mendeley.com/catalogue/4b2b7cf5-0518-3797-a68f-29c4a87a0119/

M3 - Other contribution

T3 - arXiv

PB - arXiv

ER -