Measuring intracellular motion in cancer cell using optical coherence tomography

Azhar Zam, Michael C. Kolios

Research output: Chapter in Book/Report/Conference proceedingConference contribution


In this study, we demonstrate that OCT speckle decorrelation techniques can be used to probe intracellular motion in cancer cells. Spheroids and cell pellets were used as a model to probe intracellular motion. ZnCl2 was used to inhibit mitochondrial motion within the cells. The results reveal the changes in intracellular motion during the spheroid growth phase. Moreover, to modify the motion of mitochondria, cell pellet were exposed to ZnCl2, and agent known to o impair cellular energy production through inhibition of mitochondrial function. The speckle decorrelation time during the growth phase of spheroids decreased by 35 ms over 21 days and 25 ms during inhibition of mitochondrial motion 10 minutes after exposure to ZnCl2.

Original languageEnglish (US)
Title of host publicationDynamics and Fluctuations in Biomedical Photonics XIII
EditorsValery V. Tuchin, Martin J. Leahy, Kirill V. Larin, Valery V. Tuchin, Ruikang K. Wang
ISBN (Electronic)9781628419412
StatePublished - 2016
EventDynamics and Fluctuations in Biomedical Photonics XIII Conference - San Francisco, United States
Duration: Feb 14 2016Feb 15 2016

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceDynamics and Fluctuations in Biomedical Photonics XIII Conference
Country/TerritoryUnited States
CitySan Francisco


  • Cancer cells
  • Intracellular motion
  • Mitochondria
  • Motility map
  • Optical coherence tomography
  • Speckle decorrelation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging


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