TY - JOUR
T1 - Mechanically Assisted Bioluminescence with Natural Luciferase
AU - Schramm, Stefan
AU - Al-Handawi, Marieh B.
AU - Karothu, Durga Prasad
AU - Kurlevskaya, Anastasiya
AU - Commins, Patrick
AU - Mitani, Yasuo
AU - Wu, Chun
AU - Ohmiya, Yoshihiro
AU - Naumov, Panče
N1 - Publisher Copyright:
© 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2020/9/14
Y1 - 2020/9/14
N2 - Mechanochemical analogues have recently been established for several enzymatic reactions, but they require periodic interruption of the reaction for sampling, dissolution, and (bio)chemical analysis to monitor their progress. By applying a mechanochemical procedure to induce bioluminescence analogous to that used by the marine ostracod Cypridina (Vargula) hilgendorfii, here we demonstrate that the light emitted by a bioluminescent reaction can be used to directly monitor the progress of a mechanoenzymatic reaction without sampling. Mechanical treatment of Cypridina luciferase with luciferin generates bright blue light which can be readily detected and analyzed spectroscopically. This mechanically assisted bioluminescence proceeds through a mechanism identical to that of bioluminescence in solution, but has higher activation energy due to being diffusion-controlled in the viscous matrix. The results suggest that luciferases could be used as light-emissive reporters of mechanoenzymatic reactions.
AB - Mechanochemical analogues have recently been established for several enzymatic reactions, but they require periodic interruption of the reaction for sampling, dissolution, and (bio)chemical analysis to monitor their progress. By applying a mechanochemical procedure to induce bioluminescence analogous to that used by the marine ostracod Cypridina (Vargula) hilgendorfii, here we demonstrate that the light emitted by a bioluminescent reaction can be used to directly monitor the progress of a mechanoenzymatic reaction without sampling. Mechanical treatment of Cypridina luciferase with luciferin generates bright blue light which can be readily detected and analyzed spectroscopically. This mechanically assisted bioluminescence proceeds through a mechanism identical to that of bioluminescence in solution, but has higher activation energy due to being diffusion-controlled in the viscous matrix. The results suggest that luciferases could be used as light-emissive reporters of mechanoenzymatic reactions.
KW - Cypridina
KW - bioluminescence
KW - luciferase
KW - mechanochemistry
KW - solvent-assisted reactions
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U2 - 10.1002/anie.202007440
DO - 10.1002/anie.202007440
M3 - Article
C2 - 32543104
AN - SCOPUS:85088238966
SN - 1433-7851
VL - 59
SP - 16485
EP - 16489
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 38
ER -