Mechanism of protein kinase C activation during the induction and maintenance of long-term potentiation probed using a selective peptide substrate

Eric Klann, Shu Jen Chen, J. David Sweatt

Research output: Contribution to journalArticle

Abstract

Previous reports using various protein kinase inhibitors have suggested that protein kinase activity is necessary for both the induction and maintenance of hippocampal long-term potentiation (LTP), a cellular phenomenon likely to contribute to mammalian memory formation. We designed and characterized a selective peptide substrate for protein kinase C (PKC), corresponding to amino acids 28 to 43 of the neuronal protein neurogranin, and used the substrate to obtain direct biochemical evidence for activation of PKC in both the induction and maintenance phases of LTP. As the effect cannot be accounted for by either of two well-known mechanisms for persistent PKC activation, membrane insertion, or proteolysis, the persistent activation of PKC in the maintenance phase of LTP appears to occur via another mechanism. The maintenance phase of LTP is associated with decreased immunoreactivity of PKC, an effect that can be reversed with phosphatase treatment. Thus, PKC appears to be both phosphorylated and persistently activated in the maintenance phase of LTP.

Original languageEnglish (US)
Pages (from-to)8337-8341
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number18
DOIs
StatePublished - Sep 15 1993

Keywords

  • Hippocampus
  • Phosphorylation
  • Synaptic plasticity

ASJC Scopus subject areas

  • General

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