Mechanisms of femtosecond laser cell surgery in the low-density plasma regime

K. Kuetemeyer, K. Kuetemeyer, R. Rezgui, H. Lubatschowski, A. Heisterkamp

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Although femtosecond laser cell surgery is widely used for fundamental research in cell biology, the mechanisms in the so-called low-density plasma regime are largely unknown. To date, it is still unclear on which time scales free electron and free radical-induced chemical effects take place leading to intracellular ablation. In this paper, we present our experimental study on the influence of laser parameters and staining on the ablation threshold. We found that the ablation effect resulted from the accumulation of single-shot multiphoton-induced photochemical effects finished within a few nanoseconds. In addition, fluorescence staining of subcellular structures significantly decreased the ablation threshold. Based on our findings, we propose that dye molecules are the major source for providing seed electrons for the ionization cascade.

Original languageEnglish (US)
Title of host publicationOptical Interactions with Tissue and Cells XXII
StatePublished - 2011
EventOptical Interactions with Tissue and Cells XXII - San Francisco, CA, United States
Duration: Jan 24 2011Jan 26 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherOptical Interactions with Tissue and Cells XXII
Country/TerritoryUnited States
CitySan Francisco, CA


  • Cell surgery
  • Femtosecond laser
  • Free radicals
  • Low-density plasma
  • Photochemistry

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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