@article{ae322368080c47c29605d687025c4c88,
title = "Mesoscale Modeling and Single-Nucleosome Tracking Reveal Remodeling of Clutch Folding and Dynamics in Stem Cell Differentiation",
abstract = "G{\'o}mez-Garc{\'i}a et al. show that the Pou5f1 gene folds into nucleosome clutches, with larger clutches in differentiated cells than in stem cells. These clutch changes are accompanied by enhanced hierarchical looping in differentiated cells. H2B dynamics is cell-type specific, correlates with clutch patterns, and is regulated by linker histone H1.",
keywords = "chromatin dynamics, chromatin structure, mesoscale modeling, single molecule tracking",
author = "G{\'o}mez-Garc{\'i}a, {Pablo Aurelio} and Stephanie Portillo-Ledesma and Neguembor, {Maria Victoria} and Martina Pesaresi and Walaa Oweis and Talia Rohrlich and Stefan Wieser and Eran Meshorer and Tamar Schlick and Cosma, {Maria Pia} and Melike Lakadamyali",
note = "Funding Information: This work was supported by a University of Pennsylvania Epigenetics Pilot Award (M.L.); an NSF Center for Engineering and Mechanobiology (CEMB) Pilot Award (M.L.); a Linda Pechenik Montague Investigator Award (M.L.); the European Union{\textquoteright}s Horizon 2020 Research and Innovation Programme (CellViewer grant 686637 to M.L., M.P.C., and E.M.); Ministerio de Ciencia, Innovaci{\'o}n y Universidades ( BFU2017-86760-P [AEI/FEDER, UE]) and Secretaria d{\textquoteright}Universitats i Recerca del Departament d{\textquoteright}Empresa i Coneixement de la Generalitat de Catalunya (AGAUR grant 2017 SGR 689 to M.P.C.); National NSFC grant 319711771003712 (M.P.C.); National Institutes of Health , National Institute of General Medical Sciences awards R01-GM055264 and R35-GM122562 ; Phillip-Morris USA and Phillip-Morris International (T.S.); People Program (Marie Curie Actions) FP7/2007–2013 under REA grant 608959 (M.V.N.); and Juan de la Cierva-Incorporaci{\'o}n 2017 (M.V.N.). Funding Information: This work was supported by a University of Pennsylvania Epigenetics Pilot Award (M.L.); an NSF Center for Engineering and Mechanobiology (CEMB) Pilot Award (M.L.); a Linda Pechenik Montague Investigator Award (M.L.); the European Union's Horizon 2020 Research and Innovation Programme (CellViewer grant 686637 to M.L. M.P.C. and E.M.); Ministerio de Ciencia, Innovaci?n y Universidades (BFU2017-86760-P [AEI/FEDER, UE]) and Secretaria d'Universitats i Recerca del Departament d'Empresa i Coneixement de la Generalitat de Catalunya (AGAUR grant 2017 SGR 689 to M.P.C.); National NSFC grant 319711771003712 (M.P.C.); National Institutes of Health, National Institute of General Medical Sciences awards R01-GM055264 and R35-GM122562; Phillip-Morris USA and Phillip-Morris International (T.S.); People Program (Marie Curie Actions) FP7/2007?2013 under REA grant 608959 (M.V.N.); and Juan de la Cierva-Incorporaci?n 2017 (M.V.N.). Conceptualization, M.L. and M.P.C.; Methodology, P.A.G.-G. S.P.-L. T.S. and M.L.; Modeling, S.P.L. and T.S.; Software, P.A.G.-G. S.P.-L.; Experiments, P.A.G.-G. M.V.N, M.P, W.O. and T.R.; Reagents, cell lines and other resources: M.V.N. M.P. W.O. T.R. E.M. and S.W.; Analysis, P.A.G.-G. and S.P.-L. Writing ? Original Draft: M.L. M.P.C. P.A.G.-G. and T.S.; Supervision, M.L. M.P.C. and T.S. Funding Acquisition, M.L. M.P.C. T.S. and E.M. All authors read and provided comments on the manuscript. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2020 The Author(s)",
year = "2021",
month = jan,
day = "12",
doi = "10.1016/j.celrep.2020.108614",
language = "English (US)",
volume = "34",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "2",
}