Mesoscale Modeling and Single-Nucleosome Tracking Reveal Remodeling of Clutch Folding and Dynamics in Stem Cell Differentiation

Pablo Aurelio Gómez-García, Stephanie Portillo-Ledesma, Maria Victoria Neguembor, Martina Pesaresi, Walaa Oweis, Talia Rohrlich, Stefan Wieser, Eran Meshorer, Tamar Schlick, Maria Pia Cosma, Melike Lakadamyali

Research output: Contribution to journalArticlepeer-review

Abstract

Gómez-García et al. show that the Pou5f1 gene folds into nucleosome clutches, with larger clutches in differentiated cells than in stem cells. These clutch changes are accompanied by enhanced hierarchical looping in differentiated cells. H2B dynamics is cell-type specific, correlates with clutch patterns, and is regulated by linker histone H1.

Original languageEnglish (US)
Article number108614
JournalCell Reports
Volume34
Issue number2
DOIs
StatePublished - Jan 12 2021

Keywords

  • chromatin dynamics
  • chromatin structure
  • mesoscale modeling
  • single molecule tracking

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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