TY - JOUR
T1 - Metabolic effects of some electrofluorimetric dyes
AU - Kinnally, Kathleen Walsh
AU - Tedeschi, Henry
N1 - Funding Information:
This researchw as aided by National Science Foundation Grant PCM76-23747 and National Institutes of Health Grant No. NSO7681. We thank A. Waggoner( Dept.of Chem. Amherst College,A mherst,M ass.)for supplying
PY - 1978/8/8
Y1 - 1978/8/8
N2 - The effect of five electrofluorimetric dyes on mitochondrial metabolism was examined to determine their suitability for mitochondrial studies and other biological uses. The dyes merocyanine 540, 8-anilino-1-naphthalene sulfonic acid and bis(1,3-dibutyl barbituric acid-(5))-pentamethane oxonol were found to be inhibitors of the respiratory chain. However, the first two exerted their effect only at high concentrations. 3.3′-Dihexyl-2,2′-oxacarbocyanine was found to act as an uncoupler. 3,3′-Dipropyl-thiocarbocyanine inhibited β-hydroxybutyrate respiration while dissociating succinate supported respiration from the phosphorylation of ADP. Merocyanine 540 and 8-anilino-1-naphthalene sulfonic acid may be the best suited for studies of membrane potentials in mitochondria since their effect on metabolism is negligible.
AB - The effect of five electrofluorimetric dyes on mitochondrial metabolism was examined to determine their suitability for mitochondrial studies and other biological uses. The dyes merocyanine 540, 8-anilino-1-naphthalene sulfonic acid and bis(1,3-dibutyl barbituric acid-(5))-pentamethane oxonol were found to be inhibitors of the respiratory chain. However, the first two exerted their effect only at high concentrations. 3.3′-Dihexyl-2,2′-oxacarbocyanine was found to act as an uncoupler. 3,3′-Dipropyl-thiocarbocyanine inhibited β-hydroxybutyrate respiration while dissociating succinate supported respiration from the phosphorylation of ADP. Merocyanine 540 and 8-anilino-1-naphthalene sulfonic acid may be the best suited for studies of membrane potentials in mitochondria since their effect on metabolism is negligible.
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U2 - 10.1016/0005-2728(78)90195-0
DO - 10.1016/0005-2728(78)90195-0
M3 - Article
C2 - 687611
AN - SCOPUS:0017886374
SN - 0005-2728
VL - 503
SP - 380
EP - 388
JO - BBA - Bioenergetics
JF - BBA - Bioenergetics
IS - 2
ER -