Metabolic effects of some electrofluorimetric dyes

Kathleen Walsh Kinnally; Henry Tedeschi

doi:10.1016/0005-2728(78)90195-0

Metabolic effects of some electrofluorimetric dyes

Kathleen Walsh Kinnally, Henry Tedeschi

Research output: Contribution to journal › Article › peer-review

Abstract

The effect of five electrofluorimetric dyes on mitochondrial metabolism was examined to determine their suitability for mitochondrial studies and other biological uses. The dyes merocyanine 540, 8-anilino-1-naphthalene sulfonic acid and bis(1,3-dibutyl barbituric acid-(5))-pentamethane oxonol were found to be inhibitors of the respiratory chain. However, the first two exerted their effect only at high concentrations. 3.3′-Dihexyl-2,2′-oxacarbocyanine was found to act as an uncoupler. 3,3′-Dipropyl-thiocarbocyanine inhibited β-hydroxybutyrate respiration while dissociating succinate supported respiration from the phosphorylation of ADP. Merocyanine 540 and 8-anilino-1-naphthalene sulfonic acid may be the best suited for studies of membrane potentials in mitochondria since their effect on metabolism is negligible.

Original language	English (US)
Pages (from-to)	380-388
Number of pages	9
Journal	BBA - Bioenergetics
Volume	503
Issue number	2
DOIs	https://doi.org/10.1016/0005-2728(78)90195-0
State	Published - Aug 8 1978

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

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10.1016/0005-2728(78)90195-0

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title = "Metabolic effects of some electrofluorimetric dyes",

abstract = "The effect of five electrofluorimetric dyes on mitochondrial metabolism was examined to determine their suitability for mitochondrial studies and other biological uses. The dyes merocyanine 540, 8-anilino-1-naphthalene sulfonic acid and bis(1,3-dibutyl barbituric acid-(5))-pentamethane oxonol were found to be inhibitors of the respiratory chain. However, the first two exerted their effect only at high concentrations. 3.3′-Dihexyl-2,2′-oxacarbocyanine was found to act as an uncoupler. 3,3′-Dipropyl-thiocarbocyanine inhibited β-hydroxybutyrate respiration while dissociating succinate supported respiration from the phosphorylation of ADP. Merocyanine 540 and 8-anilino-1-naphthalene sulfonic acid may be the best suited for studies of membrane potentials in mitochondria since their effect on metabolism is negligible.",

author = "Kinnally, {Kathleen Walsh} and Henry Tedeschi",

note = "Funding Information: This researchw as aided by National Science Foundation Grant PCM76-23747 and National Institutes of Health Grant No. NSO7681. We thank A. Waggoner( Dept.of Chem. Amherst College,A mherst,M ass.)for supplying",

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doi = "10.1016/0005-2728(78)90195-0",

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T1 - Metabolic effects of some electrofluorimetric dyes

AU - Kinnally, Kathleen Walsh

AU - Tedeschi, Henry

N1 - Funding Information: This researchw as aided by National Science Foundation Grant PCM76-23747 and National Institutes of Health Grant No. NSO7681. We thank A. Waggoner( Dept.of Chem. Amherst College,A mherst,M ass.)for supplying

PY - 1978/8/8

Y1 - 1978/8/8

N2 - The effect of five electrofluorimetric dyes on mitochondrial metabolism was examined to determine their suitability for mitochondrial studies and other biological uses. The dyes merocyanine 540, 8-anilino-1-naphthalene sulfonic acid and bis(1,3-dibutyl barbituric acid-(5))-pentamethane oxonol were found to be inhibitors of the respiratory chain. However, the first two exerted their effect only at high concentrations. 3.3′-Dihexyl-2,2′-oxacarbocyanine was found to act as an uncoupler. 3,3′-Dipropyl-thiocarbocyanine inhibited β-hydroxybutyrate respiration while dissociating succinate supported respiration from the phosphorylation of ADP. Merocyanine 540 and 8-anilino-1-naphthalene sulfonic acid may be the best suited for studies of membrane potentials in mitochondria since their effect on metabolism is negligible.

AB - The effect of five electrofluorimetric dyes on mitochondrial metabolism was examined to determine their suitability for mitochondrial studies and other biological uses. The dyes merocyanine 540, 8-anilino-1-naphthalene sulfonic acid and bis(1,3-dibutyl barbituric acid-(5))-pentamethane oxonol were found to be inhibitors of the respiratory chain. However, the first two exerted their effect only at high concentrations. 3.3′-Dihexyl-2,2′-oxacarbocyanine was found to act as an uncoupler. 3,3′-Dipropyl-thiocarbocyanine inhibited β-hydroxybutyrate respiration while dissociating succinate supported respiration from the phosphorylation of ADP. Merocyanine 540 and 8-anilino-1-naphthalene sulfonic acid may be the best suited for studies of membrane potentials in mitochondria since their effect on metabolism is negligible.

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U2 - 10.1016/0005-2728(78)90195-0

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AN - SCOPUS:0017886374

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SP - 380

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JO - BBA - Bioenergetics

JF - BBA - Bioenergetics

IS - 2

ER -

Metabolic effects of some electrofluorimetric dyes

Abstract

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