Metabolic effects of some electrofluorimetric dyes

Kathleen Walsh Kinnally, Henry Tedeschi

Research output: Contribution to journalArticle

Abstract

The effect of five electrofluorimetric dyes on mitochondrial metabolism was examined to determine their suitability for mitochondrial studies and other biological uses. The dyes merocyanine 540, 8-anilino-1-naphthalene sulfonic acid and bis(1,3-dibutyl barbituric acid-(5))-pentamethane oxonol were found to be inhibitors of the respiratory chain. However, the first two exerted their effect only at high concentrations. 3.3′-Dihexyl-2,2′-oxacarbocyanine was found to act as an uncoupler. 3,3′-Dipropyl-thiocarbocyanine inhibited β-hydroxybutyrate respiration while dissociating succinate supported respiration from the phosphorylation of ADP. Merocyanine 540 and 8-anilino-1-naphthalene sulfonic acid may be the best suited for studies of membrane potentials in mitochondria since their effect on metabolism is negligible.

Original languageEnglish (US)
Pages (from-to)380-388
Number of pages9
JournalBBA - Bioenergetics
Volume503
Issue number2
DOIs
StatePublished - Aug 8 1978

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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