Methods for hyaluronan molecular mass determination by agarose gel electrophoresis

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Abstract

    The average molecular mass of hyaluronan (HA) in most healthy biological fluids and tissues is usually about 6000–8000 kDa, but the biosynthetic mechanism results in a polydisperse mixture of sizes. Subsequent enzymatic degradation, or the action of reactive oxygen and nitrogen species, can further increase polydispersity and decrease the average size. Fragmented HA can be a biomarker of inflammation. In addition, reductions in HA size are associated with tissue remodeling and repair processes. Some cell-surface receptor proteins have been reported to have HA-binding affinities that are size specific, and participate in activation of signaling cascades controlling multiple aspects of cell behavior. Here we describe simple agarose gel electrophoresis protocols for the determination of the molecular mass distribution of HA isolated from tissues and fluids.

    Original languageEnglish (US)
    Title of host publicationMethods in Molecular Biology
    PublisherHumana Press Inc.
    Pages91-102
    Number of pages12
    DOIs
    StatePublished - 2019

    Publication series

    NameMethods in Molecular Biology
    Volume1952
    ISSN (Print)1064-3745

    Keywords

    • Agarose gel electrophoresis
    • Glycosaminoglycan
    • Hyaluronan
    • Molecular mass
    • Molecular mass distribution
    • Molecular weight

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

    Fingerprint Dive into the research topics of 'Methods for hyaluronan molecular mass determination by agarose gel electrophoresis'. Together they form a unique fingerprint.

  • Cite this

    Cowman, M. K. (2019). Methods for hyaluronan molecular mass determination by agarose gel electrophoresis. In Methods in Molecular Biology (pp. 91-102). (Methods in Molecular Biology; Vol. 1952). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9133-4_8