Methods for isolating and analyzing physiological hyaluronan: a review

Felipe Rivas, Dorothea Erxleben, Ian Smith, Elaheh Rahbar, Paul L. DeAngelis, Mary Cowman, Adam R. Hall

Research output: Contribution to journalReview articlepeer-review


The carbohydrate hyaluronan (or hyaluronic acid, HA) is found in all human tissues and biofluids where it has wide-ranging functions in health and disease that are dictated by both its abundance and size. Consequently, hyaluronan evaluation in physiological samples has significant translational potential. Although the analytical tools and techniques for probing other biomolecules such as proteins and nucleic acids have become standard approaches in biochemistry, those available for investigating hyaluronan are less well established. In this review, we survey methods related to the assessment of native hyaluronan in biological specimens, including protocols for separating it from biological matrices and technologies for determining its concentration and molecular weight.
Original languageEnglish (US)
Pages (from-to)C674-C687
JournalAmerican Journal of Physiology - Cell Physiology
Issue number4
StatePublished - Apr 1 2022


  • extraction
  • hyaluronan
  • quantification
  • size distribution
  • translational biomarkers

ASJC Scopus subject areas

  • Physiology
  • Cell Biology


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