@inbook{a2ea1b1fa96047efbe94a9ed6b378532,
title = "Methods for Single Cell Transcriptomic Analysis of Adipose Tissue",
abstract = "The development of single cell approaches has facilitated the investigation of cellular heterogeneity and cell type-specific gene expression in complex tissues. Adipose tissue depots contain lipid storing adipocytes as well as a diverse array of cell types that form the adipocyte niche and regulate adipose tissue function. Here, I describe two protocols for the isolation of single cells and nuclei from white and brown adipose tissue. Additionally, I provide a detailed workflow for isolation of cell type- or lineage-specific single nuclei using nuclear tagging and translating ribosome affinity purification (NuTRAP) mouse models.",
keywords = "Adipocyte progenitors, Adipose tissue, Transcriptomics, scRNA-seq, snRNA-seq",
author = "Farnaz Shamsi",
note = "Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-3167-6_21",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "241--249",
booktitle = "Methods in Molecular Biology",
}