Methods of digital video microscopy for colloidal studies

John C. Crocker, David G. Grier

    Research output: Contribution to journalArticlepeer-review


    We describe a set of image processing algorithms for extracting quantitative data from digitized video microscope images of colloidal suspensions. In a typical application, these direct imaging techniques can locate submicrometer spheres to within 10 nm in the focal plane and 150 nm in depth. Combining information from a sequence of video images into single-particle trajectories makes possible measurements of quantities of fundamental and practical interest such as diffusion coefficients and pair-wise interaction potentials. The measurements we describe in detail combine the outstanding resolution of digital imaging with video-synchronized optical trapping to obtain highly accurate and reproducible results very rapidly.

    Original languageEnglish (US)
    Pages (from-to)298-310
    Number of pages13
    JournalJournal of Colloid And Interface Science
    Issue number1
    StatePublished - Apr 15 1996


    • colloid
    • diffusion coefficient
    • dynamics
    • image processing
    • interaction potential
    • optical tweezers
    • video microscopy

    ASJC Scopus subject areas

    • Electronic, Optical and Magnetic Materials
    • Biomaterials
    • Surfaces, Coatings and Films
    • Colloid and Surface Chemistry


    Dive into the research topics of 'Methods of digital video microscopy for colloidal studies'. Together they form a unique fingerprint.

    Cite this