Micro-tweezers and Force Microscopy Techniques for Single-Cell Mechanobiological Analysis

Lanqi Gong; Weiyi Qian; Renee Tyler Tan Morales; Jie Tong; Apratim Bajpai; Weiqiang Chen

doi:10.1007/978-981-10-8953-4_39

Micro-tweezers and Force Microscopy Techniques for Single-Cell Mechanobiological Analysis

Lanqi Gong, Weiyi Qian, Renee Tyler Tan Morales, Jie Tong, Apratim Bajpai, Weiqiang Chen

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

In the past two decades, the field of cell mechanobiology has witnessed tremendous advances in bioengineering approaches for facilitating disease diagnosis and regenerative medicine. Cells can sense mechanical stimuli from their dynamic tissue microenvironment and convert them into biochemical signals that drive a wide variety of cellular behaviors and processes. Micro-tweezers have been particularly recognized as powerful tools that can stimulate and control the mechanical cues upon single cells and probe the associated cellular responses. Meanwhile, force microscopy techniques have been indispensable for quantifying and profiling cell force dynamics. This chapter discusses the principles of recently established micro-tweezers and force-sensing platforms, highlighting their capabilities, limitations, and potential integration for a single-cell mechanobiological analysis.

Original language	English (US)
Title of host publication	Handbook of Single-Cell Technologies
Publisher	Springer Singapore
Pages	1011-1032
Number of pages	22
ISBN (Electronic)	9789811089534
ISBN (Print)	9789811089527
DOIs	https://doi.org/10.1007/978-981-10-8953-4_39
State	Published - Jan 1 2021

ASJC Scopus subject areas

Engineering(all)
Biochemistry, Genetics and Molecular Biology(all)

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10.1007/978-981-10-8953-4_39

Cite this

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abstract = "In the past two decades, the field of cell mechanobiology has witnessed tremendous advances in bioengineering approaches for facilitating disease diagnosis and regenerative medicine. Cells can sense mechanical stimuli from their dynamic tissue microenvironment and convert them into biochemical signals that drive a wide variety of cellular behaviors and processes. Micro-tweezers have been particularly recognized as powerful tools that can stimulate and control the mechanical cues upon single cells and probe the associated cellular responses. Meanwhile, force microscopy techniques have been indispensable for quantifying and profiling cell force dynamics. This chapter discusses the principles of recently established micro-tweezers and force-sensing platforms, highlighting their capabilities, limitations, and potential integration for a single-cell mechanobiological analysis.",

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AU - Gong, Lanqi

AU - Qian, Weiyi

AU - Morales, Renee Tyler Tan

AU - Tong, Jie

AU - Bajpai, Apratim

AU - Chen, Weiqiang

PY - 2021/1/1

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Micro-tweezers and Force Microscopy Techniques for Single-Cell Mechanobiological Analysis

Abstract

ASJC Scopus subject areas

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