TY - JOUR
T1 - Microarray analysis of the moss Physcomitrella patens reveals evolutionarily conserved transcriptional regulation of salt stress and abscisic acid signalling
AU - Richardt, Sandra
AU - Timmerhaus, Gerrit
AU - Lang, Daniel
AU - Qudeimat, Enas
AU - Corrêa, Luiz G.G.
AU - Reski, Ralf
AU - Rensing, Stefan A.
AU - Frank, Wolfgang
N1 - Funding Information:
Acknowledgments We thank Richard Haas for technical support. This work was supported by the German Research Foundation (grants Re 837/6-3 to R.R. and W.F. and Re 837/10-2 to R.R. and S.A.R.), the Federal Ministry of Education and Research (grant FRISYS 0313921), the Excellence Initiative of the German Federal and State Governments (grant EXC 294), and the German Academic Exchange Service (Ph.D. fellowships to E.Q. and L.G.G.C.).
PY - 2010/1
Y1 - 2010/1
N2 - Regulatory networks of salt stress and abscisic acid (ABA) responses have previously been analyzed in seed plants. Here, we report microarray expression profiles of 439 genes encoding transcription-associated proteins (TAPs) in response to salt stress and ABA in the salt-tolerant moss Physcomitrella patens. Fourteen and 56 TAP genes were differentially expressed within 60 min of NaCl and ABA treatment, respectively, indicating that these responses are regulated at the transcriptional level. Overlapping expression profiles, as well as the up-regulation of ABA biosynthesis genes, suggest that ABA mediates the salt stress responses in P. patens. Comparison to public gene expression data of Arabidopsis thaliana and phylogenetic analyses suggest that the role of DREB-like, Dof, and bHLH TAPs in salt stress responses have been conserved during embryophyte evolution, and that the function of ABI3-like, bZIP, HAP3, and CO-like TAPs in seed development and flowering emerged from pre-existing ABA and light signalling pathways.
AB - Regulatory networks of salt stress and abscisic acid (ABA) responses have previously been analyzed in seed plants. Here, we report microarray expression profiles of 439 genes encoding transcription-associated proteins (TAPs) in response to salt stress and ABA in the salt-tolerant moss Physcomitrella patens. Fourteen and 56 TAP genes were differentially expressed within 60 min of NaCl and ABA treatment, respectively, indicating that these responses are regulated at the transcriptional level. Overlapping expression profiles, as well as the up-regulation of ABA biosynthesis genes, suggest that ABA mediates the salt stress responses in P. patens. Comparison to public gene expression data of Arabidopsis thaliana and phylogenetic analyses suggest that the role of DREB-like, Dof, and bHLH TAPs in salt stress responses have been conserved during embryophyte evolution, and that the function of ABI3-like, bZIP, HAP3, and CO-like TAPs in seed development and flowering emerged from pre-existing ABA and light signalling pathways.
KW - Abscisic acid
KW - Gene expression profiling
KW - NCED
KW - Physcomitrella patens
KW - Salt tolerance
KW - Transcription factors
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U2 - 10.1007/s11103-009-9550-6
DO - 10.1007/s11103-009-9550-6
M3 - Article
C2 - 19806323
AN - SCOPUS:72149091719
SN - 0167-4412
VL - 72
SP - 27
EP - 45
JO - Plant Molecular Biology
JF - Plant Molecular Biology
IS - 1-2
ER -