Microfabricated nanotopological surfaces for study of adhesion-dependent cell mechanosensitivity

Cells exhibit high sensitivity and diverse responses to the intrinsic nanotopography of the extracellular matrix through their nanoscale cellular sensing machinery. A simple microfabrication method for precise control and spatial patterning of the local nanoroughness on glass surfaces by using photolithography and reactive ion etching is reported. It is demonstrated that local nanoroughness as a biophysical cue could regulate a diverse array of NIH/3T3 fibroblast behaviors, including cell morphology, adhesion, proliferation, migration, and cytoskeleton contractility. The capability to control and further predict cellular responses to nanoroughness might suggest novel methods for developing biomaterials mimicking nanotopographic structures in vivo for functional tissue engineering. A simple method for precise control and spatial patterning of local nanoroughness on glass surfaces is reported. Nanoroughness is a potent physical signal in the cell microenvironment to regulate cell functions, including cell morphology, adhesion, proliferation, and migration. Nanotopographic sensing by cells might involve integrin-mediated adhesion signaling and cytoskeletal contractility.