Microfluidic image cytometry for quantitative single-cell profiling of human pluripotent stem cells in chemically defined conditions

Ken Ichiro Kamei; Minori Ohashi; Eric Gschweng; Quinn Ho; Jane Suh; Jinghua Tang; Zeta Tak For Yu; Amander T. Clark; April D. Pyle; Michael A. Teitell; Ki Bum Lee; Owen N. Witte; Hsian Rong Tseng

doi:10.1039/b922884e

Microfluidic image cytometry for quantitative single-cell profiling of human pluripotent stem cells in chemically defined conditions

Ken Ichiro Kamei, Minori Ohashi, Eric Gschweng, Quinn Ho, Jane Suh, Jinghua Tang, Zeta Tak For Yu, Amander T. Clark, April D. Pyle, Michael A. Teitell, Ki Bum Lee, Owen N. Witte, Hsian Rong Tseng

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Microfluidic image cytometry (MIC) has been developed to study phenotypes of various hPSC lines by screening several chemically defined serum/feeder-free conditions. A chemically defined hPSC culture was established using 20 ng mL^-1 of bFGF on 20 μg mL^-1 of Matrigel to grow hPSCs over a week in an undifferentiated state. Following hPSC culture, we conducted quantitative MIC to perform a single cell profiling of simultaneously detected protein expression (OCT4 and SSEA1). Using clustering analysis, we were able to systematically compare the characteristics of various hPSC lines in different conditions.

Original language	English (US)
Pages (from-to)	1113-1119
Number of pages	7
Journal	Lab on a Chip
Volume	10
Issue number	9
DOIs	https://doi.org/10.1039/b922884e
State	Published - 2010

ASJC Scopus subject areas

Bioengineering
Biochemistry
General Chemistry
Biomedical Engineering

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10.1039/b922884e

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Kamei, K. I., Ohashi, M., Gschweng, E., Ho, Q., Suh, J., Tang, J., For Yu, Z. T., Clark, A. T., Pyle, A. D., Teitell, M. A., Lee, K. B., Witte, O. N., & Tseng, H. R. (2010). Microfluidic image cytometry for quantitative single-cell profiling of human pluripotent stem cells in chemically defined conditions. Lab on a Chip, 10(9), 1113-1119. https://doi.org/10.1039/b922884e

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title = "Microfluidic image cytometry for quantitative single-cell profiling of human pluripotent stem cells in chemically defined conditions",

abstract = "Microfluidic image cytometry (MIC) has been developed to study phenotypes of various hPSC lines by screening several chemically defined serum/feeder-free conditions. A chemically defined hPSC culture was established using 20 ng mL-1 of bFGF on 20 μg mL-1 of Matrigel to grow hPSCs over a week in an undifferentiated state. Following hPSC culture, we conducted quantitative MIC to perform a single cell profiling of simultaneously detected protein expression (OCT4 and SSEA1). Using clustering analysis, we were able to systematically compare the characteristics of various hPSC lines in different conditions.",

author = "Kamei, {Ken Ichiro} and Minori Ohashi and Eric Gschweng and Quinn Ho and Jane Suh and Jinghua Tang and {For Yu}, {Zeta Tak} and Clark, {Amander T.} and Pyle, {April D.} and Teitell, {Michael A.} and Lee, {Ki Bum} and Witte, {Owen N.} and Tseng, {Hsian Rong}",

year = "2010",

doi = "10.1039/b922884e",

language = "English (US)",

volume = "10",

pages = "1113--1119",

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AU - Kamei, Ken Ichiro

AU - Ohashi, Minori

AU - Gschweng, Eric

AU - Ho, Quinn

AU - Suh, Jane

AU - Tang, Jinghua

AU - For Yu, Zeta Tak

AU - Clark, Amander T.

AU - Pyle, April D.

AU - Teitell, Michael A.

AU - Lee, Ki Bum

AU - Witte, Owen N.

AU - Tseng, Hsian Rong

PY - 2010

Y1 - 2010

N2 - Microfluidic image cytometry (MIC) has been developed to study phenotypes of various hPSC lines by screening several chemically defined serum/feeder-free conditions. A chemically defined hPSC culture was established using 20 ng mL-1 of bFGF on 20 μg mL-1 of Matrigel to grow hPSCs over a week in an undifferentiated state. Following hPSC culture, we conducted quantitative MIC to perform a single cell profiling of simultaneously detected protein expression (OCT4 and SSEA1). Using clustering analysis, we were able to systematically compare the characteristics of various hPSC lines in different conditions.

AB - Microfluidic image cytometry (MIC) has been developed to study phenotypes of various hPSC lines by screening several chemically defined serum/feeder-free conditions. A chemically defined hPSC culture was established using 20 ng mL-1 of bFGF on 20 μg mL-1 of Matrigel to grow hPSCs over a week in an undifferentiated state. Following hPSC culture, we conducted quantitative MIC to perform a single cell profiling of simultaneously detected protein expression (OCT4 and SSEA1). Using clustering analysis, we were able to systematically compare the characteristics of various hPSC lines in different conditions.

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Microfluidic image cytometry for quantitative single-cell profiling of human pluripotent stem cells in chemically defined conditions

Abstract

ASJC Scopus subject areas

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