Microfluidic image cytometry for single-cell phenotyping of human pluripotent stem cells

Yasumasa Mashimo, Ken Ichiro Kamei

Research output: Chapter in Book/Report/Conference proceedingChapter


A microfluidic human pluripotent stem cell (hPSC) array has been developed for robust and reproducible hPSC culture methods to assess chemically defined serum- and feeder-free culture conditions. This microfluidic platform, combined with image cytometry, enables the systematic analysis of multiple simultaneously detected marker expression in individual cells, for screening of various chemically defined media across hPSC lines, and the study of phenotypic responses.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages14
StatePublished - 2015

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Basic fibroblast growth factor
  • Chemically defined media
  • Human pluripotent stem cells
  • Immunofluorescence
  • Matrigel
  • Microfluidic hPSC array
  • Microfluidic image cytometry

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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