Microfluidic image cytometry (MIC) technology for in vitro molecular diagnostics

J. Sun; M. Ohashi; K. Kamei; H. Wang; M. Masterman-Smith; Z. T.F. Yu; H. I. Kornblum; P. S. Mischel; H. R. Tseng

Microfluidic image cytometry (MIC) technology for in vitro molecular diagnostics

J. Sun, M. Ohashi, K. Kamei, H. Wang, M. Masterman-Smith, Z. T.F. Yu, H. I. Kornblum, P. S. Mischel, H. R. Tseng

Biology

Research output: Contribution to conference › Paper › peer-review

Abstract

Microfluidic image cytometry (MIC) technology has been developed and utilized to perform quantitative/multiparameter immunocytochemistry (ICC), enabling parallel detection of multiple proteins in individual cells. A number of glioblastoma cell lines, as well as genetically modified and primary glioblastoma cells were utilized to validate the MIC technology. The dynamic changes of the proteins associated with the PI3K signaling pathway upon the exposure to exogenous stimuli (e.g., growth factors and kinase inhibitors) can be kinetically monitored, providing a potent tool to better understand how these glioblastoma cells respond to a combination of drug treatments.

Original language	English (US)
Pages	1108-1110
Number of pages	3
State	Published - 2008
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: Oct 12 2008 → Oct 16 2008

Other

Other	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	10/12/08 → 10/16/08

Keywords

Cancer diagnostics
Image cytometry
Immunocytochemistry
Signaling profile

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

Sun, J., Ohashi, M., Kamei, K., Wang, H., Masterman-Smith, M., Yu, Z. T. F., Kornblum, H. I., Mischel, P. S., & Tseng, H. R. (2008). Microfluidic image cytometry (MIC) technology for in vitro molecular diagnostics. 1108-1110. Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

Sun, J, Ohashi, M, Kamei, K, Wang, H, Masterman-Smith, M, Yu, ZTF, Kornblum, HI, Mischel, PS & Tseng, HR 2008, 'Microfluidic image cytometry (MIC) technology for in vitro molecular diagnostics', Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States, 10/12/08 - 10/16/08 pp. 1108-1110.

@conference{cbd6b760edf74008b9add7d117e3659e,

title = "Microfluidic image cytometry (MIC) technology for in vitro molecular diagnostics",

abstract = "Microfluidic image cytometry (MIC) technology has been developed and utilized to perform quantitative/multiparameter immunocytochemistry (ICC), enabling parallel detection of multiple proteins in individual cells. A number of glioblastoma cell lines, as well as genetically modified and primary glioblastoma cells were utilized to validate the MIC technology. The dynamic changes of the proteins associated with the PI3K signaling pathway upon the exposure to exogenous stimuli (e.g., growth factors and kinase inhibitors) can be kinetically monitored, providing a potent tool to better understand how these glioblastoma cells respond to a combination of drug treatments.",

keywords = "Cancer diagnostics, Image cytometry, Immunocytochemistry, Signaling profile",

author = "J. Sun and M. Ohashi and K. Kamei and H. Wang and M. Masterman-Smith and Yu, {Z. T.F.} and Kornblum, {H. I.} and Mischel, {P. S.} and Tseng, {H. R.}",

year = "2008",

language = "English (US)",

pages = "1108--1110",

note = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 ; Conference date: 12-10-2008 Through 16-10-2008",

}

TY - CONF

T1 - Microfluidic image cytometry (MIC) technology for in vitro molecular diagnostics

AU - Sun, J.

AU - Ohashi, M.

AU - Kamei, K.

AU - Wang, H.

AU - Masterman-Smith, M.

AU - Yu, Z. T.F.

AU - Kornblum, H. I.

AU - Mischel, P. S.

AU - Tseng, H. R.

PY - 2008

Y1 - 2008

N2 - Microfluidic image cytometry (MIC) technology has been developed and utilized to perform quantitative/multiparameter immunocytochemistry (ICC), enabling parallel detection of multiple proteins in individual cells. A number of glioblastoma cell lines, as well as genetically modified and primary glioblastoma cells were utilized to validate the MIC technology. The dynamic changes of the proteins associated with the PI3K signaling pathway upon the exposure to exogenous stimuli (e.g., growth factors and kinase inhibitors) can be kinetically monitored, providing a potent tool to better understand how these glioblastoma cells respond to a combination of drug treatments.

AB - Microfluidic image cytometry (MIC) technology has been developed and utilized to perform quantitative/multiparameter immunocytochemistry (ICC), enabling parallel detection of multiple proteins in individual cells. A number of glioblastoma cell lines, as well as genetically modified and primary glioblastoma cells were utilized to validate the MIC technology. The dynamic changes of the proteins associated with the PI3K signaling pathway upon the exposure to exogenous stimuli (e.g., growth factors and kinase inhibitors) can be kinetically monitored, providing a potent tool to better understand how these glioblastoma cells respond to a combination of drug treatments.

KW - Cancer diagnostics

KW - Image cytometry

KW - Immunocytochemistry

KW - Signaling profile

UR - http://www.scopus.com/inward/record.url?scp=84902465819&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84902465819&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:84902465819

SP - 1108

EP - 1110

T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

Microfluidic image cytometry (MIC) technology for in vitro molecular diagnostics

Abstract

Other

Keywords

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this