TY - GEN
T1 - MICROFLUIDIC PROBE FOR ELECTRO-PERMEABILIZATION-BASED SINGLE CELL ANALYSIS
AU - Sofela, Samuel
AU - Saleh, Alla
AU - Qasaimeh, Mohammad A.
N1 - Publisher Copyright:
© 2022 MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2022
Y1 - 2022
N2 - Spatiotemporal heterogeneity of cells within tissues play critical roles in their function, and hence, understanding heterogeneity within tissues at the single-cell level is vital. Available techniques require cells to be extracted from their physiological environment and consequently disrupt spatial resolution needed for reliable omics studies. Here, we have developed a microfluidic probe (MFP) integrated with a pair of electrodes for electropermeabilization, towards single-cell cytoplasmic biopsy and macromolecule delivery within adherent culture.
AB - Spatiotemporal heterogeneity of cells within tissues play critical roles in their function, and hence, understanding heterogeneity within tissues at the single-cell level is vital. Available techniques require cells to be extracted from their physiological environment and consequently disrupt spatial resolution needed for reliable omics studies. Here, we have developed a microfluidic probe (MFP) integrated with a pair of electrodes for electropermeabilization, towards single-cell cytoplasmic biopsy and macromolecule delivery within adherent culture.
KW - 3D printing
KW - biopsy
KW - electropermeabilization
KW - Integrated microfluidic probe
KW - single-cell analysis
UR - http://www.scopus.com/inward/record.url?scp=85175688680&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85175688680&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85175688680
T3 - MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 454
EP - 455
BT - MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022
Y2 - 23 October 2022 through 27 October 2022
ER -