Microfluidic Probe for Multiphysics Enabled Single Cell Manipulation

Ayoola Brimmo, Mohammad A. Qasaimeh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this work, we develop microfluidic probes (MFPs) by utilizing high resolution 3D printing, and demonstrate applications in targeted drug delivery with a single cell resolution. In addition, the MFP's features are extended to include heating elements in order to facilitate its application as a single cell micro-thermo-fluidic tweezer - a heat assisted microfluidic single cell tweezer. With the high resolution 2photon photolithography 3D printing technique (Nanoscribe), we achieve microfluidic injection apertures of 30 \mu \mathrm{m} diameter which corresponds to the average span of single cells in adherent culture. Flow characterization demonstrates the devices' capability of rapidly adjusting its localized target area from about 100 cells (32,000 \mu \mathrm{m}^{2}) to single cell resolutions (320 \mu \mathrm{m}^{2}). Results show that these resolutions can be maintained in the scanning mode to achieve high resolution drug delivery in cultured adherent cells. Furthermore, as an advancement to the conventional MFP, pin shape electrodes were introduced at the tip to incorporate localized heating features for optimal cell dissociation conditions. Characterization of the heat generated by the pin electrodes is reported and an application of the micro-thermo-fluidic tweezer to selectively detach and collect single adherent cells is demonstrated.

Original languageEnglish (US)
Title of host publicationProceedings of MARSS 2020
Subtitle of host publicationInternational Conference on Manipulation, Automation, and Robotics at Small Scales
EditorsSinan Haliyo, Mokrane Boudaoud, Albert Sill, Sergej Fatikow
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781728193496
DOIs
StatePublished - Jul 13 2020
Event2020 International Conference on Manipulation, Automation, and Robotics at Small Scales, MARSS 2020 - Toronto, Canada
Duration: Jul 13 2020Jul 17 2020

Publication series

NameProceedings of MARSS 2020: International Conference on Manipulation, Automation, and Robotics at Small Scales

Conference

Conference2020 International Conference on Manipulation, Automation, and Robotics at Small Scales, MARSS 2020
CountryCanada
CityToronto
Period7/13/207/17/20

Keywords

  • 3D printing
  • Biological cells
  • Joule heating
  • Micro-manipulation
  • Microfluidic probe
  • Microfluidics
  • Tweezer

ASJC Scopus subject areas

  • Fluid Flow and Transfer Processes
  • Artificial Intelligence
  • Mechanical Engineering
  • Control and Optimization

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