@inproceedings{c842f28a2d8a42b583d646a141f0f671,
title = "Microfluidic system for pulsed stimulation and time course analysis of mammalian cells: Identification of the minimal TNF-alpha pulse duration for NF-KAPPAB activation in HeLa cells",
abstract = "We present a simple hydrostatic-driven microfluidic system for spatiotemporal cell stimulation. We used the developed system to culture HeLa cancer cells and apply stimulation pulses of Tumor necrosis factor (TNF) as short as 10 s. Using this system, we established the concentration-dependent temporal limit of TNF-stimulated NF-kB activation in HeLa cells, and determined that a 30-seconds pulse is sufficient for full activation of NF-kB with 100 ng/ml TNF, and that a 1-min pulse is needed with 10 ng/ml TNF.",
keywords = "HeLa cells, Hydrostatic-driven flow, Multiple laminar streams, Pulsatile stimulation, Tumor necrosis factor",
author = "Qasaimeh, {M. A.} and R. Lee and S. Gaudet and D. Juncker",
year = "2012",
language = "English (US)",
isbn = "9780979806452",
series = "Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012",
publisher = "Chemical and Biological Microsystems Society",
pages = "1075--1077",
booktitle = "Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012",
note = "16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 ; Conference date: 28-10-2012 Through 01-11-2012",
}