Microfluidic system for pulsed stimulation and time course analysis of mammalian cells: Identification of the minimal TNF-alpha pulse duration for NF-KAPPAB activation in HeLa cells

M. A. Qasaimeh, R. Lee, S. Gaudet, D. Juncker

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a simple hydrostatic-driven microfluidic system for spatiotemporal cell stimulation. We used the developed system to culture HeLa cancer cells and apply stimulation pulses of Tumor necrosis factor (TNF) as short as 10 s. Using this system, we established the concentration-dependent temporal limit of TNF-stimulated NF-kB activation in HeLa cells, and determined that a 30-seconds pulse is sufficient for full activation of NF-kB with 100 ng/ml TNF, and that a 1-min pulse is needed with 10 ng/ml TNF.

Original languageEnglish (US)
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1075-1077
Number of pages3
ISBN (Print)9780979806452
StatePublished - 2012
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: Oct 28 2012Nov 1 2012

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period10/28/1211/1/12

Keywords

  • HeLa cells
  • Hydrostatic-driven flow
  • Multiple laminar streams
  • Pulsatile stimulation
  • Tumor necrosis factor

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Qasaimeh, M. A., Lee, R., Gaudet, S., & Juncker, D. (2012). Microfluidic system for pulsed stimulation and time course analysis of mammalian cells: Identification of the minimal TNF-alpha pulse duration for NF-KAPPAB activation in HeLa cells. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 1075-1077). (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012). Chemical and Biological Microsystems Society.