Micromechanical elastomeric devices for investigations of mechanobiology in human embryonic stem cells

Yubing Sun, Luis G. Villa-Diaz, Raymond H.W. Lam, Weiqiang Chen, Paul H. Krebsbach, Jianping Fu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Human embryonic stem cells (hESCs) provided a cell source for biotechnological and clinical applications. Yet, there is limited understanding of how mechanical signals in the microenvironment of hESCs regulate their fate decisions. Here, we applied a microfabricated micromechanical platform to investigate mechanoresponsive behaviors of hESCs. We demonstrated that hESCs are mechanosensitive, and they could increase their cytoskeleton contractility with matrix rigidity. Furthermore, rigid substrates supported maintenance of pluripotency of hESCs. Matrix mechanics-mediated cytoskeleton contractility of hESCs might be functionally correlated with E-cadherin expressions in cell-cell contacts and thus involved in fate decisions of hESCs. Our results provided a novel approach to characterize and understand mechanotransduction in hESC function regulation.

Original languageEnglish (US)
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1714-1716
Number of pages3
ISBN (Print)9780979806452
StatePublished - 2012
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: Oct 28 2012Nov 1 2012

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period10/28/1211/1/12

Keywords

  • Cell mechanics
  • Human embryonic stem cells
  • Mechanotransduction
  • Microfabrication
  • Pluripotency

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Sun, Y., Villa-Diaz, L. G., Lam, R. H. W., Chen, W., Krebsbach, P. H., & Fu, J. (2012). Micromechanical elastomeric devices for investigations of mechanobiology in human embryonic stem cells. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 1714-1716). (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012). Chemical and Biological Microsystems Society.