TY - GEN
T1 - Microscale Fluidic Manipulation on a Paper Platform for Rapid Multiplex Detection of SARS-CoV-2 Genes
AU - Sukumar, Pavithra
AU - Saleh, Alla
AU - Deliorman, Muhammedin
AU - Qasaimeh, Mohammad A.
N1 - Publisher Copyright:
© 2024 IEEE.
PY - 2024
Y1 - 2024
N2 - Here, we developed a novel paper-based microfluidic point-of-care diagnostic platform for multiplex detection of viral pathogen SARS-CoV-2 genes.The platform utilizes reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay technology.Paper-based assay optimizations were carried out leading to achievement of a color readout at 10 copies/µL RNA sensitivity.We also demonstrated singleplex detection on microspots using colorimetric pH-based dye (phenol red), with pink indicating negative and yellow indicating positive results.Single-layered device is designed with radial quadrants for multiplexing, narrow fluidic resistors for preventing backflow, and fluidic ports for venting excess fluids.With our device, multiplex detection of various SARS-CoV-2 genes is possible.Moreover, we demonstrate rapid qualitative detection of positive or negative results in 2 and 4 min.In addition, to improve the visual detection of acidic pH yellow color on paper, we utilized in-situ gold nanoparticles (AuNP) synthesis on microspots, resulting in a pinkish-purple color change based on the concentration of the amplicon.The developed platform can be easily modified and optimized to detect other pathogens, such as viruses and bacteria, in both singleplex and multiplexed formats.
AB - Here, we developed a novel paper-based microfluidic point-of-care diagnostic platform for multiplex detection of viral pathogen SARS-CoV-2 genes.The platform utilizes reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay technology.Paper-based assay optimizations were carried out leading to achievement of a color readout at 10 copies/µL RNA sensitivity.We also demonstrated singleplex detection on microspots using colorimetric pH-based dye (phenol red), with pink indicating negative and yellow indicating positive results.Single-layered device is designed with radial quadrants for multiplexing, narrow fluidic resistors for preventing backflow, and fluidic ports for venting excess fluids.With our device, multiplex detection of various SARS-CoV-2 genes is possible.Moreover, we demonstrate rapid qualitative detection of positive or negative results in 2 and 4 min.In addition, to improve the visual detection of acidic pH yellow color on paper, we utilized in-situ gold nanoparticles (AuNP) synthesis on microspots, resulting in a pinkish-purple color change based on the concentration of the amplicon.The developed platform can be easily modified and optimized to detect other pathogens, such as viruses and bacteria, in both singleplex and multiplexed formats.
KW - AuNPs
KW - Colorimetric point-of-care diagnostics
KW - Multiplexing
KW - Paper microfluidics
KW - RT-LAMP
KW - SARS-CoV-2
UR - http://www.scopus.com/inward/record.url?scp=85202343919&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85202343919&partnerID=8YFLogxK
U2 - 10.1109/MARSS61851.2024.10612751
DO - 10.1109/MARSS61851.2024.10612751
M3 - Conference contribution
AN - SCOPUS:85202343919
T3 - Proceedings of MARSS 2024 - 7th International Conference on Manipulation, Automation, and Robotics at Small Scales
BT - Proceedings of MARSS 2024 - 7th International Conference on Manipulation, Automation, and Robotics at Small Scales
A2 - Haliyo, Sinan
A2 - Boudaoud, Mokrane
A2 - Mastrangeli, Massimo
A2 - Lambert, Pierre
A2 - Fatikow, Sergej
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 7th International Conference on Manipulation, Automation, and Robotics at Small Scales, MARSS 2024
Y2 - 1 July 2024 through 5 July 2024
ER -