Mitochondrial nitric-oxide synthase: Enzyme expression, characterization, and regulation

Virginia Haynes, Sarah Elfering, Nathaniel Traaseth, Cecilia Giulivi

Research output: Contribution to journalArticlepeer-review


Nitric oxide is generated in vivo by nitric-oxide synthase (NOS) during the conversion of L-Arg to citrulline. Using a variety of biological systems and approaches emerging evidence has been accumulated for the occurrence of a mitochondrial NOS (mtNOS), identified as the alpha isoform of neuronal or NOS-1. Under physiological conditions, the production of nitric oxide by mitochondria has an important implication for the maintenance of the cellular metabolism, i.e. modulates the oxygen consumption of the organelles through the competitive (with oxygen) and reversible inhibition of cytochrome c oxidase. The transient inhibition suits the continuously changing energy and oxygen requirements of the tissue; it is a short-term regulation with profound pathophysiological consequences. This review describes the identification of mtNOS and the role of posttranslational modifications on mtNOS' activity and regulation.

Original languageEnglish (US)
Pages (from-to)341-346
Number of pages6
JournalJournal of Bioenergetics and Biomembranes
Issue number4 SPEC.ISS.
StatePublished - Aug 2004


  • Nitric oxide
  • mitochondria
  • nitric-oxide synthase
  • oxygen
  • oxygen consumption
  • posttranslational modifications

ASJC Scopus subject areas

  • Physiology
  • Cell Biology


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