Modern techniques for the analysis of chromatin and nuclear organization in C. elegans

Peter Askjaer, Sevinç Ercan, Peter Meister

Research output: Contribution to journalReview articlepeer-review

Abstract

In recent years, Caenorhabditis elegans has emerged as a new model to investigate the relationships between nuclear architecture, cellular differentiation, and organismal development. On one hand, C. elegans with its fixed lineage and transparent body is a great model organism to observe gene functions in vivo in specific cell types using microscopy. On the other hand, two different techniques have been applied in nematodes to identify binding sites for chromatin-associated proteins genome-wide: chromatin immunoprecipitation (ChIP), and Dam-mediated identification (DamID). We summarize here all three techniques together as they are complementary. We also highlight strengths and differences of the individual approaches.

Original languageEnglish (US)
Pages (from-to)1-35
Number of pages35
JournalWormBook : the online review of C. elegans biology
DOIs
StatePublished - 2014

ASJC Scopus subject areas

  • Medicine(all)

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