@article{1579f5f40eab4035a9c6141cd670657c,
title = "Molecular architecture of SMC proteins and the yeast cohesin complex",
abstract = "Sister chromatids are held together by the multisubunit cohesin complex, which contains two SMC (Smc1 and Smc3) and two non-SMC (Scc1 and Scc3) proteins. The crystal structure of a bacterial SMC {"}hinge{"} region along with EM studies and biochemical experiments on yeast Smc1 and Smc3 proteins show that SMC protamers fold up individually into rod-shaped molecules. A 45 nm long intramolecular coiled coil separates the hinge region from the ATPase-containing {"}head{"} domain. Smc1 and Smc3 bind to each other via heterotypic interactions between their hinges to form a V-shaped heterodimer. The two heads of the V-shaped dimer are connected by different ends of the cleavable Scc1 subunit. Cohesin therefore forms a large proteinaceous loop within which sister chromatids might be entrapped after DNA replication.",
author = "Haering, {Christian H.} and Jan L{\"o}we and Andreas Hochwagen and Kim Nasmyth",
note = "Funding Information: We are very grateful to F. Uhlmann for extensive experimental advice and many helpful suggestions at the outset of this project. We thank C. Frei and S. Gasser (Lausanne) for providing the Smc1 antibody, D. Schoffnegger and K. Tachibana for experimental assistance, A. Toth and R. Ciosk for yeast strain construction, P. Steinlein, I. Fischer, and S. Reipert for help with BIAcore and electron microscopy, K. Mechtler for mass-spectrometry, J.M. Peters and M. Glotzer for comments on the manuscript, and the members of the Nasmyth and Peters labs for helpful discussions. We are very grateful to James Nicholson (beamline 9.5, SRS, Daresbury Laboratories, UK) for help with MAD data collection. We would also like to thank the staff at beamlines 14.1 (SRS) and ID14-4 of ESRF (ESRF, Grenoble, France) for assistance with data collection. This research was supported by Boehringer Ingelheim International, the Austrian Industrial Research Promotion Fund (FFF), and the Austrian Science Fund (FWF). ",
year = "2002",
doi = "10.1016/S1097-2765(02)00515-4",
language = "English (US)",
volume = "9",
pages = "773--788",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "4",
}