Molecular cloning, expression and potential functions of the human proteinase-activated receptor-2

Stephan K. Böhm, Wuyi Kong, Dieter Brömme, Steven P. Smeekens, David C. Anderson, Andrew Connolly, Mark Kahn, Nicholas A. Nelken, Shaun R. Coughlin, Donald G. Payan, Nigel W. Bunnett

Research output: Contribution to journalArticle

Abstract

We used PCR to amplify proteinase activated receptor-2 (PAR-2) from human kidney cDNA. The open reading frame comprised 1191 bp and encoded a protein of 397 residues with 83% identify with mouse PAR-2. In KNRK cells (a line of kirsten murine sarcoma virus-transformed rat kidney epithelial cells) transfected with this cDNA, trypsin and activating peptide (AP) corresponding to the tethered ligand exposed by trypsin cleavage (SLIGKV-NH2) induced a prompt increase in cytosolic calcium ion concentration ([Ca2+](i)). Human PAR-2 (hPAR-2) resided both on the plasma membrane and in the Golgi apparatus. hPAR-2 mRNA was highly expressed in human pancreas, kidney, colon, liver and small intestine, and by A549 lung and SW480 colon adenocarcinoma cells. Hybridization in situ revealed high expression in intestinal epithelial cells throughout the gut. Trypsin and AP stimulated an increase in [Ca2+], in a rat intestinal epithelial cell line (hBRIE 380) and stimulated amylase secretion in isolated pancreatic acini. In A549 cells, which also responded to trypsin and AP with mobilization of cytosolic Ca2+, AP inhibited colony formation. Thus PAR-2 may serve as a trypsin sensor in the gut. Its expression by cells and tissues not normally exposed to pancreatic trypsin suggests that other proteases could serve as physiological activators.

Original languageEnglish (US)
Pages (from-to)1009-1016
Number of pages8
JournalBiochemical Journal
Volume314
Issue number3
DOIs
StatePublished - Mar 15 1996

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Böhm, S. K., Kong, W., Brömme, D., Smeekens, S. P., Anderson, D. C., Connolly, A., Kahn, M., Nelken, N. A., Coughlin, S. R., Payan, D. G., & Bunnett, N. W. (1996). Molecular cloning, expression and potential functions of the human proteinase-activated receptor-2. Biochemical Journal, 314(3), 1009-1016. https://doi.org/10.1042/bj3141009