Molecular mechanism for eliminylation, a newly discovered post-translational modification

Zhihong Ke, Gregory K. Smith, Yingkai Zhang, Hua Guo

Research output: Contribution to journalArticle

Abstract

The newly discovered bacterial phosphothreonine lyases perform a post-translational modification of host cell signaling proteins through a novel catalytic mechanism that irreversibly removes the phosphate group from a phosphorylated threonine via β-elimination. This "eliminylation" reaction is shown by ab initio QM/MM studies to proceed via an E1cB-like pathway, in which the carbanion intermediate is stabilized by an enzyme oxyanion hole provided by Lys104 and Tyr158 of SpvC.

Original languageEnglish (US)
Pages (from-to)11103-11105
Number of pages3
JournalJournal of the American Chemical Society
Volume133
Issue number29
DOIs
StatePublished - Jul 27 2011

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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