Monkey-MIMMS: Towards Automated Cellular Resolution Large- Scale Two-Photon Microscopy in the Awake Macaque Monkey

John Choi; Vasily Goncharov; Jessica Kleinbart; Amy Orsborn; Bijan Pesaran

doi:10.1109/EMBC.2018.8512994

Monkey-MIMMS: Towards Automated Cellular Resolution Large- Scale Two-Photon Microscopy in the Awake Macaque Monkey

John Choi, Vasily Goncharov, Jessica Kleinbart, Amy Orsborn, Bijan Pesaran

Neural Science

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The size and curvature of the macaque brain present challenges for two photon laser scanning microscopy (2P-LSM). General access to the cortex requires 5-axis positioning over a range of motion wider than existing designs offer. In addition, movement artifacts due to physiological pulsations and bodily movement present particular challenges. We present a microscope and implant platform that allows for repeatable, motorized positioning and stable imaging at any point on the dorsal convexity of macaque cortex. While testing the system to image neurons expressing fluorescent proteins in an awake macaque, motion artifacts were limited to several microns.

Original language	English (US)
Title of host publication	40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	3013-3016
Number of pages	4
ISBN (Electronic)	9781538636466
DOIs	https://doi.org/10.1109/EMBC.2018.8512994
State	Published - Oct 26 2018
Event	40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018 - Honolulu, United States Duration: Jul 18 2018 → Jul 21 2018

Publication series

Name	Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
Volume	2018-July
ISSN (Print)	1557-170X

Other

Other	40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018
Country/Territory	United States
City	Honolulu
Period	7/18/18 → 7/21/18

ASJC Scopus subject areas

Signal Processing
Biomedical Engineering
Computer Vision and Pattern Recognition
Health Informatics

Access to Document

10.1109/EMBC.2018.8512994

Cite this

Choi, J., Goncharov, V., Kleinbart, J., Orsborn, A., & Pesaran, B. (2018). Monkey-MIMMS: Towards Automated Cellular Resolution Large- Scale Two-Photon Microscopy in the Awake Macaque Monkey. In 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018 (pp. 3013-3016). Article 8512994 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS; Vol. 2018-July). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/EMBC.2018.8512994

Monkey-MIMMS: Towards Automated Cellular Resolution Large- Scale Two-Photon Microscopy in the Awake Macaque Monkey. / Choi, John; Goncharov, Vasily; Kleinbart, Jessica et al.
40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018. Institute of Electrical and Electronics Engineers Inc., 2018. p. 3013-3016 8512994 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS; Vol. 2018-July).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Choi, J, Goncharov, V, Kleinbart, J, Orsborn, A & Pesaran, B 2018, Monkey-MIMMS: Towards Automated Cellular Resolution Large- Scale Two-Photon Microscopy in the Awake Macaque Monkey. in 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018., 8512994, Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS, vol. 2018-July, Institute of Electrical and Electronics Engineers Inc., pp. 3013-3016, 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018, Honolulu, United States, 7/18/18. https://doi.org/10.1109/EMBC.2018.8512994

Choi J, Goncharov V, Kleinbart J, Orsborn A, Pesaran B. Monkey-MIMMS: Towards Automated Cellular Resolution Large- Scale Two-Photon Microscopy in the Awake Macaque Monkey. In 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018. Institute of Electrical and Electronics Engineers Inc. 2018. p. 3013-3016. 8512994. (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS). doi: 10.1109/EMBC.2018.8512994

Choi, John ; Goncharov, Vasily ; Kleinbart, Jessica et al. / Monkey-MIMMS : Towards Automated Cellular Resolution Large- Scale Two-Photon Microscopy in the Awake Macaque Monkey. 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018. Institute of Electrical and Electronics Engineers Inc., 2018. pp. 3013-3016 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS).

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Monkey-MIMMS: Towards Automated Cellular Resolution Large- Scale Two-Photon Microscopy in the Awake Macaque Monkey

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