Monkey-MIMMS: Towards Automated Cellular Resolution Large- Scale Two-Photon Microscopy in the Awake Macaque Monkey

John Choi, Vasily Goncharov, Jessica Kleinbart, Amy Orsborn, Bijan Pesaran

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The size and curvature of the macaque brain present challenges for two photon laser scanning microscopy (2P-LSM). General access to the cortex requires 5-axis positioning over a range of motion wider than existing designs offer. In addition, movement artifacts due to physiological pulsations and bodily movement present particular challenges. We present a microscope and implant platform that allows for repeatable, motorized positioning and stable imaging at any point on the dorsal convexity of macaque cortex. While testing the system to image neurons expressing fluorescent proteins in an awake macaque, motion artifacts were limited to several microns.

Original languageEnglish (US)
Title of host publication40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages3013-3016
Number of pages4
ISBN (Electronic)9781538636466
DOIs
StatePublished - Oct 26 2018
Event40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018 - Honolulu, United States
Duration: Jul 18 2018Jul 21 2018

Publication series

NameProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
Volume2018-July
ISSN (Print)1557-170X

Other

Other40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018
CountryUnited States
CityHonolulu
Period7/18/187/21/18

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

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    Choi, J., Goncharov, V., Kleinbart, J., Orsborn, A., & Pesaran, B. (2018). Monkey-MIMMS: Towards Automated Cellular Resolution Large- Scale Two-Photon Microscopy in the Awake Macaque Monkey. In 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018 (pp. 3013-3016). [8512994] (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS; Vol. 2018-July). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/EMBC.2018.8512994