Multilayer scaffolds in orthopaedic tissue engineering

Kivanc Atesok, M. Nedim Doral, Jon Karlsson, Kenneth A. Egol, Laith M. Jazrawi, Paulo G. Coelho, Amaury Martinez, Tomoyuki Matsumoto, Brett D. Owens, Mitsuo Ochi, Shepard R. Hurwitz, Anthony Atala, Freddie H. Fu, Helen H. Lu, Scott A. Rodeo

Research output: Contribution to journalReview articlepeer-review


Purpose: The purpose of this study was to summarize the recent developments in the field of tissue engineering as they relate to multilayer scaffold designs in musculoskeletal regeneration. Methods: Clinical and basic research studies that highlight the current knowledge and potential future applications of the multilayer scaffolds in orthopaedic tissue engineering were evaluated and the best evidence collected. Studies were divided into three main categories based on tissue types and interfaces for which multilayer scaffolds were used to regenerate: bone, osteochondral junction and tendon-to-bone interfaces. Results: In vitro and in vivo studies indicate that the use of stratified scaffolds composed of multiple layers with distinct compositions for regeneration of distinct tissue types within the same scaffold and anatomic location is feasible. This emerging tissue engineering approach has potential applications in regeneration of bone defects, osteochondral lesions and tendon-to-bone interfaces with successful basic research findings that encourage clinical applications. Conclusions: Present data supporting the advantages of the use of multilayer scaffolds as an emerging strategy in musculoskeletal tissue engineering are promising, however, still limited. Positive impacts of the use of next generation scaffolds in orthopaedic tissue engineering can be expected in terms of decreasing the invasiveness of current grafting techniques used for reconstruction of bone and osteochondral defects, and tendon-to-bone interfaces in near future.

Original languageEnglish (US)
Pages (from-to)2365-2373
Number of pages9
JournalKnee Surgery, Sports Traumatology, Arthroscopy
Issue number7
StatePublished - Jul 1 2016


  • Interface tissue regeneration
  • Multi-lineage cell differentiation
  • Multilayer scaffolds
  • Orthopaedic tissue engineering

ASJC Scopus subject areas

  • Surgery
  • Orthopedics and Sports Medicine


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