Multilayered Microfluidic Device for Controllable Flow Perfusion of Gut-Liver on a Chip

Jiandong Yang; Satoshi Imamura; Yoshikazu Hirai; Ken Ichiro Kamei; Toshiyuki Tsuchiya; Osamu Tabata

doi:10.1109/Transducers50396.2021.9495477

Multilayered Microfluidic Device for Controllable Flow Perfusion of Gut-Liver on a Chip

Jiandong Yang, Satoshi Imamura, Yoshikazu Hirai, Ken Ichiro Kamei, Toshiyuki Tsuchiya, Osamu Tabata

Biology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Gut-liver-on-chip is useful as an in vitro model to predict drug absorption and metabolism, and to elucidate physiological mechanisms. While understanding of complex gut-live interactions requires both physiologically relevant medium perfusions and organotypic structure, none of the gut-liver-on-chip filling above requirements have been realized in a single chip. Here, we report a novel multilayered microfluidic device to reproduce the gut-liver interaction. To realize the physiological environment in vivo more realistically, compartmentalized perfusable cell chambers with a porous membrane and on-chip micropump were monolithically embedded in a single chip by means of multilayer soft lithography. Our experiments highlight that the present device can improve cell viabilities and recapitulate the gut-liver interaction, which allow to obtain deeper insights into the underlying liver disease.

Original language	English (US)
Title of host publication	21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	176-179
Number of pages	4
ISBN (Electronic)	9781665412674
DOIs	https://doi.org/10.1109/Transducers50396.2021.9495477
State	Published - Jun 20 2021
Event	21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021 - Virtual, Online, United States Duration: Jun 20 2021 → Jun 25 2021

Publication series

Name	21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021

Conference

Conference	21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021
Country/Territory	United States
City	Virtual, Online
Period	6/20/21 → 6/25/21

Keywords

Body-on-a-chip
Gut-liver axis model
Microfluidic device
Multilayer soft lithography
Poly-dimethylsiloxane

ASJC Scopus subject areas

Electrical and Electronic Engineering
Mechanical Engineering
Control and Optimization
Instrumentation

Access to Document

10.1109/Transducers50396.2021.9495477

Cite this

Yang, J., Imamura, S., Hirai, Y., Kamei, K. I., Tsuchiya, T., & Tabata, O. (2021). Multilayered Microfluidic Device for Controllable Flow Perfusion of Gut-Liver on a Chip. In 21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021 (pp. 176-179). (21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/Transducers50396.2021.9495477

Multilayered Microfluidic Device for Controllable Flow Perfusion of Gut-Liver on a Chip. / Yang, Jiandong; Imamura, Satoshi; Hirai, Yoshikazu et al.
21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021. Institute of Electrical and Electronics Engineers Inc., 2021. p. 176-179 (21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Yang, J, Imamura, S, Hirai, Y, Kamei, KI, Tsuchiya, T & Tabata, O 2021, Multilayered Microfluidic Device for Controllable Flow Perfusion of Gut-Liver on a Chip. in 21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021. 21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021, Institute of Electrical and Electronics Engineers Inc., pp. 176-179, 21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021, Virtual, Online, United States, 6/20/21. https://doi.org/10.1109/Transducers50396.2021.9495477

Yang J, Imamura S, Hirai Y, Kamei KI, Tsuchiya T, Tabata O. Multilayered Microfluidic Device for Controllable Flow Perfusion of Gut-Liver on a Chip. In 21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021. Institute of Electrical and Electronics Engineers Inc. 2021. p. 176-179. (21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021). doi: 10.1109/Transducers50396.2021.9495477

Yang, Jiandong ; Imamura, Satoshi ; Hirai, Yoshikazu et al. / Multilayered Microfluidic Device for Controllable Flow Perfusion of Gut-Liver on a Chip. 21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021. Institute of Electrical and Electronics Engineers Inc., 2021. pp. 176-179 (21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021).

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AU - Tsuchiya, Toshiyuki

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AB - Gut-liver-on-chip is useful as an in vitro model to predict drug absorption and metabolism, and to elucidate physiological mechanisms. While understanding of complex gut-live interactions requires both physiologically relevant medium perfusions and organotypic structure, none of the gut-liver-on-chip filling above requirements have been realized in a single chip. Here, we report a novel multilayered microfluidic device to reproduce the gut-liver interaction. To realize the physiological environment in vivo more realistically, compartmentalized perfusable cell chambers with a porous membrane and on-chip micropump were monolithically embedded in a single chip by means of multilayer soft lithography. Our experiments highlight that the present device can improve cell viabilities and recapitulate the gut-liver interaction, which allow to obtain deeper insights into the underlying liver disease.

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Multilayered Microfluidic Device for Controllable Flow Perfusion of Gut-Liver on a Chip

Abstract

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Keywords

ASJC Scopus subject areas

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