TY - JOUR
T1 - Multiple conductance levels in rat heart inner mitochondrial membranes studied by patch clamping
AU - Zorow, Dmitry B.
AU - Kinnally, Kathleen W.
AU - Perini, Sean
AU - Tedeschi, Henry
N1 - Funding Information:
This study was funded in part by grants from NSF DCB-8818432 and USIA IA-AEMP-GSI9339. ~;.
PY - 1992/4/13
Y1 - 1992/4/13
N2 - The behavior of the mitochondrial inner membrane multiple conductance channel (MCC) which has a peak conductance of 1-1.5 nS has been examined in rat heart mitochondria, MCC can display several unique characteristics: (a) prolonged open and closed times on the order of seconds to minutes, (b) a voltage dependence in which MCC opens (negative potential) or closes (positive potential) generally in steps, (c) a response to inhibitors such as amiodarone in steps corresponding at least approximately to those in (b), (d) a 'free-running mode' in which the current level rapidly fluctuates between a minimum of nine conductance levels but with a preferred occupation of the 0.5-0.7 nS levels, and (e) very large transitions (1-1.5 nS) resolved at 4 kHz bandwidth as single events with variable mean open time.
AB - The behavior of the mitochondrial inner membrane multiple conductance channel (MCC) which has a peak conductance of 1-1.5 nS has been examined in rat heart mitochondria, MCC can display several unique characteristics: (a) prolonged open and closed times on the order of seconds to minutes, (b) a voltage dependence in which MCC opens (negative potential) or closes (positive potential) generally in steps, (c) a response to inhibitors such as amiodarone in steps corresponding at least approximately to those in (b), (d) a 'free-running mode' in which the current level rapidly fluctuates between a minimum of nine conductance levels but with a preferred occupation of the 0.5-0.7 nS levels, and (e) very large transitions (1-1.5 nS) resolved at 4 kHz bandwidth as single events with variable mean open time.
KW - (Rat heart)
KW - Amiodarone
KW - Ion channel
KW - Mitochondrial channel
KW - Mitochondrial membrane
KW - Patch clamp
KW - Permeability
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U2 - 10.1016/0005-2736(92)90203-X
DO - 10.1016/0005-2736(92)90203-X
M3 - Article
C2 - 1586662
AN - SCOPUS:0026547650
VL - 1105
SP - 263
EP - 270
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
SN - 0005-2736
IS - 2
ER -