Multiple conductance levels in rat heart inner mitochondrial membranes studied by patch clamping

Dmitry B. Zorow; Kathleen W. Kinnally; Sean Perini; Henry Tedeschi

doi:10.1016/0005-2736(92)90203-X

Multiple conductance levels in rat heart inner mitochondrial membranes studied by patch clamping

Dmitry B. Zorow, Kathleen W. Kinnally, Sean Perini, Henry Tedeschi

Research output: Contribution to journal › Article › peer-review

Abstract

The behavior of the mitochondrial inner membrane multiple conductance channel (MCC) which has a peak conductance of 1-1.5 nS has been examined in rat heart mitochondria, MCC can display several unique characteristics: (a) prolonged open and closed times on the order of seconds to minutes, (b) a voltage dependence in which MCC opens (negative potential) or closes (positive potential) generally in steps, (c) a response to inhibitors such as amiodarone in steps corresponding at least approximately to those in (b), (d) a 'free-running mode' in which the current level rapidly fluctuates between a minimum of nine conductance levels but with a preferred occupation of the 0.5-0.7 nS levels, and (e) very large transitions (1-1.5 nS) resolved at 4 kHz bandwidth as single events with variable mean open time.

Original language	English (US)
Pages (from-to)	263-270
Number of pages	8
Journal	BBA - Biomembranes
Volume	1105
Issue number	2
DOIs	https://doi.org/10.1016/0005-2736(92)90203-X
State	Published - Apr 13 1992

Keywords

(Rat heart)
Amiodarone
Ion channel
Mitochondrial channel
Mitochondrial membrane
Patch clamp
Permeability

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

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10.1016/0005-2736(92)90203-X

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@article{df11db215a574caea667a4ec579aa5d4,

title = "Multiple conductance levels in rat heart inner mitochondrial membranes studied by patch clamping",

abstract = "The behavior of the mitochondrial inner membrane multiple conductance channel (MCC) which has a peak conductance of 1-1.5 nS has been examined in rat heart mitochondria, MCC can display several unique characteristics: (a) prolonged open and closed times on the order of seconds to minutes, (b) a voltage dependence in which MCC opens (negative potential) or closes (positive potential) generally in steps, (c) a response to inhibitors such as amiodarone in steps corresponding at least approximately to those in (b), (d) a 'free-running mode' in which the current level rapidly fluctuates between a minimum of nine conductance levels but with a preferred occupation of the 0.5-0.7 nS levels, and (e) very large transitions (1-1.5 nS) resolved at 4 kHz bandwidth as single events with variable mean open time.",

keywords = "(Rat heart), Amiodarone, Ion channel, Mitochondrial channel, Mitochondrial membrane, Patch clamp, Permeability",

author = "Zorow, {Dmitry B.} and Kinnally, {Kathleen W.} and Sean Perini and Henry Tedeschi",

note = "Funding Information: This study was funded in part by grants from NSF DCB-8818432 and USIA IA-AEMP-GSI9339. ~;.",

year = "1992",

month = apr,

day = "13",

doi = "10.1016/0005-2736(92)90203-X",

language = "English (US)",

volume = "1105",

pages = "263--270",

journal = "BBA - Biomembranes",

issn = "0005-2736",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Multiple conductance levels in rat heart inner mitochondrial membranes studied by patch clamping

AU - Zorow, Dmitry B.

AU - Kinnally, Kathleen W.

AU - Perini, Sean

AU - Tedeschi, Henry

N1 - Funding Information: This study was funded in part by grants from NSF DCB-8818432 and USIA IA-AEMP-GSI9339. ~;.

PY - 1992/4/13

Y1 - 1992/4/13

N2 - The behavior of the mitochondrial inner membrane multiple conductance channel (MCC) which has a peak conductance of 1-1.5 nS has been examined in rat heart mitochondria, MCC can display several unique characteristics: (a) prolonged open and closed times on the order of seconds to minutes, (b) a voltage dependence in which MCC opens (negative potential) or closes (positive potential) generally in steps, (c) a response to inhibitors such as amiodarone in steps corresponding at least approximately to those in (b), (d) a 'free-running mode' in which the current level rapidly fluctuates between a minimum of nine conductance levels but with a preferred occupation of the 0.5-0.7 nS levels, and (e) very large transitions (1-1.5 nS) resolved at 4 kHz bandwidth as single events with variable mean open time.

AB - The behavior of the mitochondrial inner membrane multiple conductance channel (MCC) which has a peak conductance of 1-1.5 nS has been examined in rat heart mitochondria, MCC can display several unique characteristics: (a) prolonged open and closed times on the order of seconds to minutes, (b) a voltage dependence in which MCC opens (negative potential) or closes (positive potential) generally in steps, (c) a response to inhibitors such as amiodarone in steps corresponding at least approximately to those in (b), (d) a 'free-running mode' in which the current level rapidly fluctuates between a minimum of nine conductance levels but with a preferred occupation of the 0.5-0.7 nS levels, and (e) very large transitions (1-1.5 nS) resolved at 4 kHz bandwidth as single events with variable mean open time.

KW - (Rat heart)

KW - Amiodarone

KW - Ion channel

KW - Mitochondrial channel

KW - Mitochondrial membrane

KW - Patch clamp

KW - Permeability

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U2 - 10.1016/0005-2736(92)90203-X

DO - 10.1016/0005-2736(92)90203-X

M3 - Article

C2 - 1586662

AN - SCOPUS:0026547650

SN - 0005-2736

VL - 1105

SP - 263

EP - 270

JO - BBA - Biomembranes

JF - BBA - Biomembranes

IS - 2

ER -

Multiple conductance levels in rat heart inner mitochondrial membranes studied by patch clamping

Abstract

Keywords

ASJC Scopus subject areas

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