Multiplexed proteomic sample preconcentration device using surface-patterned ion-selective membrane

Jeong Hoon Lee, Yong Ak Song, Jongyoon Han

Research output: Contribution to journalArticle

Abstract

In this paper, we report a new method of fabricating a high-throughput protein preconcentrator in poly(dimethylsiloxane) (PDMS) microfluidic chip format. We print a submicron thick ion-selective membrane on the glass substrate by using standard patterning techniques. By simply plasma-bonding a PDMS microfluidic device on top of the printed glass substrate, we can integrate the ion-selective membrane into the device and rapidly prototype a PDMS preconcentrator without complicated microfabrication and cumbersome integration processes. The PDMS preconcentrator shows a concentration factor as high as ∼104 in 5 min. This printing method even allows fabricating a parallel array of preconcentrators to increase the concentrated sample volume, which can facilitate an integration of our microfluidic preconcentrator chip as a signal enhancing tool to various detectors such as a mass spectrometer.

Original languageEnglish (US)
Pages (from-to)596-601
Number of pages6
JournalLab on a Chip
Volume8
Issue number4
DOIs
StatePublished - 2008

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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