Multiresolution identification of germ layer components in teratomas derived from human and nonhuman primate embryonic stem cells

Amina Chebira, John A. Ozolek, Carlos A. Castro, William G. Jenkinson, Mukta Gore, Ramamurthy Bhagavatula, Irina Khaimovich, Shauna E. Ormon, Christopher S. Navara, Meena Sukhwani, Kyle E. Orwig, Ahmi Ben-Yehudah, Gerald Schatten, Gustavo K. Rohde, Jelena Kovačević

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We propose a system for identification of germ layer components in teratomas derived from human and nonhuman primate embryonic stem cells. Tissue regeneration and repair, drug testing and discovery, the cure of genetic and developmental syndromes all may rest on the understanding of the biology and behavior of embryonic stem (ES) cells. Within the field of stem cell biology, an ES cell is not considered an ES cell until it can produce a teratoma tumor (the "gold" standard test); a seemingly disorganized mass of tissue derived from all three embryonic germ layers; ectoderm, mesoderm, and endoderm. Identification and quantification of tissue types within teratomas derived from ES cells may expand our knowledge of abnormal and normal developmental programming and the response of ES cells to genetic manipulation and/or toxic exposures. In addition, because of the tissue complexity, identifying and quantifying the tissue is tedious and time consuming, but in turn the teratoma provides an excellent biological platform to test robust image analysis algorithms. We use a multiresolution (MR) classification system with texture features, as well as develop novel nuclear texture features to recognize germ layer components. With redundant MR transform, we achieve a classification accuracy of approximately 88%.

Original languageEnglish (US)
Title of host publication2008 5th IEEE International Symposium on Biomedical Imaging
Subtitle of host publicationFrom Nano to Macro, Proceedings, ISBI
Pages979-982
Number of pages4
DOIs
StatePublished - 2008
Event2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI - Paris, France
Duration: May 14 2008May 17 2008

Publication series

Name2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, Proceedings, ISBI

Other

Other2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI
CountryFrance
CityParis
Period5/14/085/17/08

Keywords

  • Classification
  • Feature extraction
  • Multiresolution
  • Stem cell biology

ASJC Scopus subject areas

  • Biomedical Engineering

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