Multiresolution multiscale active mask segmentation of fluorescence microscope images

Gowri Srinivasa, Matthew Fickus, Jelena Kovacević

Research output: Chapter in Book/Report/Conference proceedingConference contribution


We propose an active mask segmentation framework that combines the advantages of statistical modeling, smoothing, speed and flexibility offered by the traditional methods of region-growing, multiscale, multiresolution and active contours respectively. At the crux of this framework is a paradigm shift from evolving contours in the continuous domain to evolving multiple masks in the discrete domain. Thus, the active mask framework is particularly suited to segment digital images. We demonstrate the use of the framework in practice through the segmentation of punctate patterns in fluorescence microscope images. Experiments reveal that statistical modeling helps the multiple masks converge from a random initial configuration to a meaningful one. This obviates the need for an involved initialization procedure germane to most of the traditional methods used to segment fluorescence microscope images. While we provide the mathematical details of the functions used to segment fluorescence microscope images, this is only an instantiation of the active mask framework. We suggest some other instantiations of the framework to segment different types of images.

Original languageEnglish (US)
Title of host publicationWavelets XIII
StatePublished - 2009
EventWavelets XIII - San Diego, CA, United States
Duration: Aug 2 2009Aug 4 2009

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X


OtherWavelets XIII
Country/TerritoryUnited States
CitySan Diego, CA


  • Active contours
  • Active masks
  • Cellular automata
  • Fluorescence microscopy
  • Multiresolution
  • Multiscale
  • Segmentation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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