Mutagenic activity and identification of excreted platinum in human and rat urine and rat plasma after administration of cisplatin

R. Safirstein; M. Daye; Joseph B. Guttenplan

doi:10.1016/0304-3835(83)90244-6

Mutagenic activity and identification of excreted platinum in human and rat urine and rat plasma after administration of cisplatin

R. Safirstein, M. Daye, Joseph B. Guttenplan

Molecular Pathobiology

Research output: Contribution to journal › Article › peer-review

Abstract

Cisplatin and its biotransformation products were analyzed in human and rat urine and in plasma from rats. Analyses were performed using high performance liquid chromatography (HPLC). Microbial mutagenesis assays were performed on effluents from the chromatographic system. After intravenous administration to man (50 mg/m²) and intravenous and intraperitoneal administration to rats (5-10 mg/kg), platinum was excreted in the urine in a form that co-eluted mainly with cisplatin. Unbound drug in the plasma co-eluted with cisplatin. Furthermore excreted platinum exhibited mutagenic and chemical reactivity similar to that of cisplatin. We conclude that the principal form of free platinum circulating in blood and excreted in urine is cisplatin.

Original language	English (US)
Pages (from-to)	329-338
Number of pages	10
Journal	Cancer Letters
Volume	18
Issue number	3
DOIs	https://doi.org/10.1016/0304-3835(83)90244-6
State	Published - Apr 1983

ASJC Scopus subject areas

Oncology
Cancer Research

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@article{20bf7dea89904ad6af3d93429e05152a,

title = "Mutagenic activity and identification of excreted platinum in human and rat urine and rat plasma after administration of cisplatin",

abstract = "Cisplatin and its biotransformation products were analyzed in human and rat urine and in plasma from rats. Analyses were performed using high performance liquid chromatography (HPLC). Microbial mutagenesis assays were performed on effluents from the chromatographic system. After intravenous administration to man (50 mg/m2) and intravenous and intraperitoneal administration to rats (5-10 mg/kg), platinum was excreted in the urine in a form that co-eluted mainly with cisplatin. Unbound drug in the plasma co-eluted with cisplatin. Furthermore excreted platinum exhibited mutagenic and chemical reactivity similar to that of cisplatin. We conclude that the principal form of free platinum circulating in blood and excreted in urine is cisplatin.",

author = "R. Safirstein and M. Daye and Guttenplan, {Joseph B.}",

note = "Funding Information: We wish to thank Dr. Fred S. Wright, Yale University School of Medicine, West Haven VA Hospital who made available the use of the atomic absorption spectrometer and Dr. Howard Bruckner, Mount Sinai School of Medicine, for providing patient{\textquoteright}s urine. We wish to thank Ms. Lilia Shoichet for her expert technical assistance and Ms. Ricky Herskovitz and Ms. Helen Phillips for preparation of the manuscript. This investigation was supported by PHS grant number lROlCA/AM28683-OlAl awarded by the National Cancer Institute, DHHS and Grant number K-064 awarded by the Health Research Council of New York.",

year = "1983",

month = apr,

doi = "10.1016/0304-3835(83)90244-6",

language = "English (US)",

volume = "18",

pages = "329--338",

journal = "Cancer Letters",

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publisher = "Elsevier Ireland Ltd",

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T1 - Mutagenic activity and identification of excreted platinum in human and rat urine and rat plasma after administration of cisplatin

AU - Safirstein, R.

AU - Daye, M.

AU - Guttenplan, Joseph B.

N1 - Funding Information: We wish to thank Dr. Fred S. Wright, Yale University School of Medicine, West Haven VA Hospital who made available the use of the atomic absorption spectrometer and Dr. Howard Bruckner, Mount Sinai School of Medicine, for providing patient’s urine. We wish to thank Ms. Lilia Shoichet for her expert technical assistance and Ms. Ricky Herskovitz and Ms. Helen Phillips for preparation of the manuscript. This investigation was supported by PHS grant number lROlCA/AM28683-OlAl awarded by the National Cancer Institute, DHHS and Grant number K-064 awarded by the Health Research Council of New York.

PY - 1983/4

Y1 - 1983/4

N2 - Cisplatin and its biotransformation products were analyzed in human and rat urine and in plasma from rats. Analyses were performed using high performance liquid chromatography (HPLC). Microbial mutagenesis assays were performed on effluents from the chromatographic system. After intravenous administration to man (50 mg/m2) and intravenous and intraperitoneal administration to rats (5-10 mg/kg), platinum was excreted in the urine in a form that co-eluted mainly with cisplatin. Unbound drug in the plasma co-eluted with cisplatin. Furthermore excreted platinum exhibited mutagenic and chemical reactivity similar to that of cisplatin. We conclude that the principal form of free platinum circulating in blood and excreted in urine is cisplatin.

AB - Cisplatin and its biotransformation products were analyzed in human and rat urine and in plasma from rats. Analyses were performed using high performance liquid chromatography (HPLC). Microbial mutagenesis assays were performed on effluents from the chromatographic system. After intravenous administration to man (50 mg/m2) and intravenous and intraperitoneal administration to rats (5-10 mg/kg), platinum was excreted in the urine in a form that co-eluted mainly with cisplatin. Unbound drug in the plasma co-eluted with cisplatin. Furthermore excreted platinum exhibited mutagenic and chemical reactivity similar to that of cisplatin. We conclude that the principal form of free platinum circulating in blood and excreted in urine is cisplatin.

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JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

IS - 3

ER -

Mutagenic activity and identification of excreted platinum in human and rat urine and rat plasma after administration of cisplatin

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