NADPH oxidase immunoreactivity in the mouse brain

Faridis Serrano; Nutan S. Kolluri; Frans B. Wientjes; J. Patrick Card; Eric Klann

doi:10.1016/S0006-8993(03)03364-X

NADPH oxidase immunoreactivity in the mouse brain

Faridis Serrano, Nutan S. Kolluri, Frans B. Wientjes, J. Patrick Card, Eric Klann

Neural Science

Research output: Contribution to journal › Article › peer-review

Abstract

Superoxide production via NADPH oxidase has been shown to play a role in neurotoxicity, ischemic stroke, and possibly Parkinson's and Alzheimer's diseases. In addition, NADPH oxidase-dependent production of superoxide may be necessary for normal brain functions, including neuronal differentiation and neuronal plasticity. To improve our understanding of NADPH oxidase in the brain, we studied the localization of the various protein components of NADPH oxidase in the central nervous system of the adult mouse using immunohistochemistry. We detected staining for the cytoplasmic NADPH proteins, p40^phox, p47^phox, and p67^phox, as well as the membrane-associated NADPH oxidase proteins, p22^phox and gp91^phox in neurons throughout the mouse brain. Staining of each of the NADPH oxidase proteins was observed in neurons in all regions of the neuraxis, with particularly prominent localizations in the hippocampus, cortex, amygdala, striatum, and thalamus. The expression of NADPH oxidase proteins in neurons suggests the possibility that enzymatic production of superoxide by a NADPH oxidase may play a role in both normal neuronal function as well as neurodegeneration in the brain.

Original language	English (US)
Pages (from-to)	193-198
Number of pages	6
Journal	Brain Research
Volume	988
Issue number	1-2
DOIs	https://doi.org/10.1016/S0006-8993(03)03364-X
State	Published - Oct 24 2003

Keywords

Hippocampus
Long-term potentiation
Neurodegeneration
Neurotoxicity
Rac
Superoxide

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/S0006-8993(03)03364-X

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title = "NADPH oxidase immunoreactivity in the mouse brain",

abstract = "Superoxide production via NADPH oxidase has been shown to play a role in neurotoxicity, ischemic stroke, and possibly Parkinson's and Alzheimer's diseases. In addition, NADPH oxidase-dependent production of superoxide may be necessary for normal brain functions, including neuronal differentiation and neuronal plasticity. To improve our understanding of NADPH oxidase in the brain, we studied the localization of the various protein components of NADPH oxidase in the central nervous system of the adult mouse using immunohistochemistry. We detected staining for the cytoplasmic NADPH proteins, p40phox, p47phox, and p67phox, as well as the membrane-associated NADPH oxidase proteins, p22phox and gp91phox in neurons throughout the mouse brain. Staining of each of the NADPH oxidase proteins was observed in neurons in all regions of the neuraxis, with particularly prominent localizations in the hippocampus, cortex, amygdala, striatum, and thalamus. The expression of NADPH oxidase proteins in neurons suggests the possibility that enzymatic production of superoxide by a NADPH oxidase may play a role in both normal neuronal function as well as neurodegeneration in the brain.",

keywords = "Hippocampus, Long-term potentiation, Neurodegeneration, Neurotoxicity, Rac, Superoxide",

author = "Faridis Serrano and Kolluri, {Nutan S.} and Wientjes, {Frans B.} and Card, {J. Patrick} and Eric Klann",

note = "Funding Information: This work was supported NIH grant NS34007 (E.K.).",

year = "2003",

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doi = "10.1016/S0006-8993(03)03364-X",

language = "English (US)",

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AU - Serrano, Faridis

AU - Kolluri, Nutan S.

AU - Wientjes, Frans B.

AU - Card, J. Patrick

AU - Klann, Eric

N1 - Funding Information: This work was supported NIH grant NS34007 (E.K.).

PY - 2003/10/24

Y1 - 2003/10/24

N2 - Superoxide production via NADPH oxidase has been shown to play a role in neurotoxicity, ischemic stroke, and possibly Parkinson's and Alzheimer's diseases. In addition, NADPH oxidase-dependent production of superoxide may be necessary for normal brain functions, including neuronal differentiation and neuronal plasticity. To improve our understanding of NADPH oxidase in the brain, we studied the localization of the various protein components of NADPH oxidase in the central nervous system of the adult mouse using immunohistochemistry. We detected staining for the cytoplasmic NADPH proteins, p40phox, p47phox, and p67phox, as well as the membrane-associated NADPH oxidase proteins, p22phox and gp91phox in neurons throughout the mouse brain. Staining of each of the NADPH oxidase proteins was observed in neurons in all regions of the neuraxis, with particularly prominent localizations in the hippocampus, cortex, amygdala, striatum, and thalamus. The expression of NADPH oxidase proteins in neurons suggests the possibility that enzymatic production of superoxide by a NADPH oxidase may play a role in both normal neuronal function as well as neurodegeneration in the brain.

AB - Superoxide production via NADPH oxidase has been shown to play a role in neurotoxicity, ischemic stroke, and possibly Parkinson's and Alzheimer's diseases. In addition, NADPH oxidase-dependent production of superoxide may be necessary for normal brain functions, including neuronal differentiation and neuronal plasticity. To improve our understanding of NADPH oxidase in the brain, we studied the localization of the various protein components of NADPH oxidase in the central nervous system of the adult mouse using immunohistochemistry. We detected staining for the cytoplasmic NADPH proteins, p40phox, p47phox, and p67phox, as well as the membrane-associated NADPH oxidase proteins, p22phox and gp91phox in neurons throughout the mouse brain. Staining of each of the NADPH oxidase proteins was observed in neurons in all regions of the neuraxis, with particularly prominent localizations in the hippocampus, cortex, amygdala, striatum, and thalamus. The expression of NADPH oxidase proteins in neurons suggests the possibility that enzymatic production of superoxide by a NADPH oxidase may play a role in both normal neuronal function as well as neurodegeneration in the brain.

KW - Hippocampus

KW - Long-term potentiation

KW - Neurodegeneration

KW - Neurotoxicity

KW - Rac

KW - Superoxide

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NADPH oxidase immunoreactivity in the mouse brain

Abstract

Keywords

ASJC Scopus subject areas

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