Nano-bio-chips for high performance multiplexed protein detection: Determinations of cancer biomarkers in serum and saliva using quantum dot bioconjugate labels

Jesse V. Jokerst, Archana Raamanathan, Nicolaos Christodoulides, Pierre N. Floriano, Amanda A. Pollard, Glennon W. Simmons, Jorge Wong, Carole Gage, Wieslaw B. Furmaga, Spencer W. Redding, John T. McDevitt

Research output: Contribution to journalArticle

Abstract

The integration of semiconductor nanoparticle quantum dots (QDs) into a modular, microfluidic biosensor for the multiplexed quantitation of three important cancer markers, carcinoembryonic antigen (CEA), cancer antigen 125 (CA125), and Her-2/Neu (C-erbB-2) was achieved. The functionality of the integrated sample processing, analyte capture and detection modalities was demonstrated using both serum and whole saliva specimens. Here, nano-bio-chips that employed a fluorescence transduction signal with QD-labeled detecting antibody were used in combination with antigen capture by a microporous agarose bead array supported within a microfluidics ensemble so as to complete the sandwich-type immunoassay. The utilization of QD probes in this miniaturized biosensor format resulted in signal amplification 30 times relative to that of standard molecular fluorophores as well as affording a reduction in observed limits of detection by nearly 2 orders of magnitude (0.02 ng/mL CEA; 0.11 pM CEA) relative to enzyme-linked immunosorbent assay (ELISA). Assay validation studies indicate that measurements by the nano-bio-chip system correlate to standard methods at R2 = 0.94 and R2 = 0.95 for saliva and serum, respectively. This integrated nano-bio-chip assay system, in tandem with next-generation fluorophores, promises to be a sensitive, multiplexed tool for important diagnostic and prognostic applications.

Original languageEnglish (US)
Pages (from-to)3622-3629
Number of pages8
JournalBiosensors and Bioelectronics
Volume24
Issue number12
DOIs
StatePublished - Aug 15 2009

Keywords

  • Cancer biomarkers
  • Lab-on-a-chip
  • Nano-bio-chip
  • Point-of-care
  • Quantum dots
  • Salivary diagnostics

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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  • Cite this

    Jokerst, J. V., Raamanathan, A., Christodoulides, N., Floriano, P. N., Pollard, A. A., Simmons, G. W., Wong, J., Gage, C., Furmaga, W. B., Redding, S. W., & McDevitt, J. T. (2009). Nano-bio-chips for high performance multiplexed protein detection: Determinations of cancer biomarkers in serum and saliva using quantum dot bioconjugate labels. Biosensors and Bioelectronics, 24(12), 3622-3629. https://doi.org/10.1016/j.bios.2009.05.026