TY - JOUR
T1 - Nano-bio-chips for high performance multiplexed protein detection
T2 - Determinations of cancer biomarkers in serum and saliva using quantum dot bioconjugate labels
AU - Jokerst, Jesse V.
AU - Raamanathan, Archana
AU - Christodoulides, Nicolaos
AU - Floriano, Pierre N.
AU - Pollard, Amanda A.
AU - Simmons, Glennon W.
AU - Wong, Jorge
AU - Gage, Carole
AU - Furmaga, Wieslaw B.
AU - Redding, Spencer W.
AU - McDevitt, John T.
N1 - Funding Information:
The authors acknowledge funding provided by the National Institutes of Health through the National Institute of Dental and Craniofacial Research (U01 DE017793). The content is solely the responsibility of the authors and does not necessarily represent or reflect views of the NIH or the Federal Government. Support was also provided by a private gift to UTHSCSA.
PY - 2009/8/15
Y1 - 2009/8/15
N2 - The integration of semiconductor nanoparticle quantum dots (QDs) into a modular, microfluidic biosensor for the multiplexed quantitation of three important cancer markers, carcinoembryonic antigen (CEA), cancer antigen 125 (CA125), and Her-2/Neu (C-erbB-2) was achieved. The functionality of the integrated sample processing, analyte capture and detection modalities was demonstrated using both serum and whole saliva specimens. Here, nano-bio-chips that employed a fluorescence transduction signal with QD-labeled detecting antibody were used in combination with antigen capture by a microporous agarose bead array supported within a microfluidics ensemble so as to complete the sandwich-type immunoassay. The utilization of QD probes in this miniaturized biosensor format resulted in signal amplification 30 times relative to that of standard molecular fluorophores as well as affording a reduction in observed limits of detection by nearly 2 orders of magnitude (0.02 ng/mL CEA; 0.11 pM CEA) relative to enzyme-linked immunosorbent assay (ELISA). Assay validation studies indicate that measurements by the nano-bio-chip system correlate to standard methods at R2 = 0.94 and R2 = 0.95 for saliva and serum, respectively. This integrated nano-bio-chip assay system, in tandem with next-generation fluorophores, promises to be a sensitive, multiplexed tool for important diagnostic and prognostic applications.
AB - The integration of semiconductor nanoparticle quantum dots (QDs) into a modular, microfluidic biosensor for the multiplexed quantitation of three important cancer markers, carcinoembryonic antigen (CEA), cancer antigen 125 (CA125), and Her-2/Neu (C-erbB-2) was achieved. The functionality of the integrated sample processing, analyte capture and detection modalities was demonstrated using both serum and whole saliva specimens. Here, nano-bio-chips that employed a fluorescence transduction signal with QD-labeled detecting antibody were used in combination with antigen capture by a microporous agarose bead array supported within a microfluidics ensemble so as to complete the sandwich-type immunoassay. The utilization of QD probes in this miniaturized biosensor format resulted in signal amplification 30 times relative to that of standard molecular fluorophores as well as affording a reduction in observed limits of detection by nearly 2 orders of magnitude (0.02 ng/mL CEA; 0.11 pM CEA) relative to enzyme-linked immunosorbent assay (ELISA). Assay validation studies indicate that measurements by the nano-bio-chip system correlate to standard methods at R2 = 0.94 and R2 = 0.95 for saliva and serum, respectively. This integrated nano-bio-chip assay system, in tandem with next-generation fluorophores, promises to be a sensitive, multiplexed tool for important diagnostic and prognostic applications.
KW - Cancer biomarkers
KW - Lab-on-a-chip
KW - Nano-bio-chip
KW - Point-of-care
KW - Quantum dots
KW - Salivary diagnostics
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UR - http://www.scopus.com/inward/citedby.url?scp=67650034253&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2009.05.026
DO - 10.1016/j.bios.2009.05.026
M3 - Article
C2 - 19576756
AN - SCOPUS:67650034253
SN - 0956-5663
VL - 24
SP - 3622
EP - 3629
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 12
ER -