TY - JOUR
T1 - Nanometer-scale features on micrometer-scale surface texturing
T2 - A bone histological, gene expression, and nanomechanical study
AU - Coelho, Paulo G.
AU - Takayama, Tadahiro
AU - Yoo, Daniel
AU - Jimbo, Ryo
AU - Karunagaran, Sanjay
AU - Tovar, Nick
AU - Janal, Malvin N.
AU - Yamano, Seiichi
N1 - Funding Information:
This study was partially supported by Intra-Lock International, Boca Raton, Florida, the American College of Prosthodontists Education Foundation–Research Fellowship in Prosthodontics, The Northeastern Gnathological Society–Granger-Pruden Award, and The Academy of Prosthodontics–Research Grant Award to Sanjay Karunagaran.
PY - 2014/8
Y1 - 2014/8
N2 - Micro- and nanoscale surface modifications have been the focus of multiple studies in the pursuit of accelerating bone apposition or osseointegration at the implant surface. Here, we evaluated histological and nanomechanical properties, and gene expression, for a microblasted surface presenting nanometer-scale texture within a micrometer-scale texture (MB) (Ossean™ Surface, Intra-Lock International, Boca Raton, FL) versus a dual-acid etched surface presenting texture at the micrometer-scale only (AA), in a rodent femur model for 1, 2, 4, and 8. weeks in vivo. Following animal sacrifice, samples were evaluated in terms of histomorphometry, biomechanical properties through nanoindentation, and gene expression by real-time quantitative reverse transcription polymerase chain reaction analysis. Although the histomorphometric, and gene expression analysis results were not significantly different between MB and AA at 4 and 8. weeks, significant differences were seen at 1 and 2. weeks. The expression of the genes encoding collagen type I (COL-1), and osteopontin (OPN) was significantly higher for MB than for AA at 1. week, indicating up-regulated osteoprogenitor and osteoblast differentiation. At 2. weeks, significantly up-regulated expression of the genes for COL-1, runt-related transcription factor 2 (RUNX-2), osterix, and osteocalcin (OCN) indicated progressive mineralization in newly formed bone. The nanomechanical properties tested by the nanoindentation presented significantly higher-rank hardness and elastic modulus for the MB compared to AA at all time points tested. In conclusion, the nanotopographical featured surfaces presented an overall higher host-to-implant response compared to the microtextured only surfaces. The statistical differences observed in some of the osteogenic gene expression between the two groups may shed some insight into the role of surface texture and its extent in the observed bone healing mechanisms.
AB - Micro- and nanoscale surface modifications have been the focus of multiple studies in the pursuit of accelerating bone apposition or osseointegration at the implant surface. Here, we evaluated histological and nanomechanical properties, and gene expression, for a microblasted surface presenting nanometer-scale texture within a micrometer-scale texture (MB) (Ossean™ Surface, Intra-Lock International, Boca Raton, FL) versus a dual-acid etched surface presenting texture at the micrometer-scale only (AA), in a rodent femur model for 1, 2, 4, and 8. weeks in vivo. Following animal sacrifice, samples were evaluated in terms of histomorphometry, biomechanical properties through nanoindentation, and gene expression by real-time quantitative reverse transcription polymerase chain reaction analysis. Although the histomorphometric, and gene expression analysis results were not significantly different between MB and AA at 4 and 8. weeks, significant differences were seen at 1 and 2. weeks. The expression of the genes encoding collagen type I (COL-1), and osteopontin (OPN) was significantly higher for MB than for AA at 1. week, indicating up-regulated osteoprogenitor and osteoblast differentiation. At 2. weeks, significantly up-regulated expression of the genes for COL-1, runt-related transcription factor 2 (RUNX-2), osterix, and osteocalcin (OCN) indicated progressive mineralization in newly formed bone. The nanomechanical properties tested by the nanoindentation presented significantly higher-rank hardness and elastic modulus for the MB compared to AA at all time points tested. In conclusion, the nanotopographical featured surfaces presented an overall higher host-to-implant response compared to the microtextured only surfaces. The statistical differences observed in some of the osteogenic gene expression between the two groups may shed some insight into the role of surface texture and its extent in the observed bone healing mechanisms.
KW - Gene expression
KW - Nanomechanics
KW - Nanotopography
KW - Osseointegration
UR - http://www.scopus.com/inward/record.url?scp=84901059817&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901059817&partnerID=8YFLogxK
U2 - 10.1016/j.bone.2014.05.004
DO - 10.1016/j.bone.2014.05.004
M3 - Article
C2 - 24813260
AN - SCOPUS:84901059817
SN - 8756-3282
VL - 65
SP - 25
EP - 32
JO - Bone
JF - Bone
ER -