Nanoplasmonic Sandwich Immunoassay for Tumor-Derived Exosome Detection and Exosomal PD-L1 Profiling

Chuanyu Wang; Chung Hui Huang; Zhuangqiang Gao; Jialiang Shen; Jiacheng He; Alana MacLachlan; Chao Ma; Ya Chang; Wen Yang; Yuxin Cai; Yang Lou; Siyuan Dai; Weiqiang Chen; Feng Li; Pengyu Chen

doi:10.1021/acssensors.1c01101

Nanoplasmonic Sandwich Immunoassay for Tumor-Derived Exosome Detection and Exosomal PD-L1 Profiling

Chuanyu Wang, Chung Hui Huang, Zhuangqiang Gao, Jialiang Shen, Jiacheng He, Alana MacLachlan, Chao Ma, Ya Chang, Wen Yang, Yuxin Cai, Yang Lou, Siyuan Dai, Weiqiang Chen, Feng Li, Pengyu Chen

Research output: Contribution to journal › Article › peer-review

Abstract

Tumor-derived exosomes play a vital role in the process of cancer development. Quantitative analysis of exosomes and exosome-shuttled proteins would be of immense value in understanding cancer progression and generating reliable predictive biomarkers for cancer diagnosis and treatment. Recent studies have indicated the critical role of exosomal programmed death ligand 1 (PD-L1) in immune checkpoint therapy and its application as a patient stratification biomarker in cancer immunotherapy. Here, we present a nanoplasmonic exosome immunoassay utilizing gold-silver (Au@Ag) core-shell nanobipyramids and gold nanorods, which form sandwich immune complexes with target exosomes. The immunoassay generates a distinct plasmonic signal pattern unique to exosomes with specific exosomal PD-L1 expression, allowing rapid, highly sensitive exosome detection and accurate identification of PD-L1 exosome subtypes in a single assay. The developed nanoplasmonic sandwich immunoassay provides a novel and viable approach for tumor cell-derived exosome detection and analysis with quantitative molecular details of key exosomal proteins, manifesting its great potential as a transformative diagnostic tool for early cancer detection, prognosis, and post-treatment monitoring.

Original language	English (US)
Pages (from-to)	3308-3319
Number of pages	12
Journal	ACS Sensors
Volume	6
Issue number	9
DOIs	https://doi.org/10.1021/acssensors.1c01101
State	Published - Sep 24 2021

Keywords

exosomal protein
exosomes
gold−silver core−shell nanobipyramids (Au@Ag NBPs)
immunoassay
localized surface plasmon resonance (LSPR)
microfluidics
programmed death ligand 1 (PD-L1)

ASJC Scopus subject areas

Bioengineering
Instrumentation
Process Chemistry and Technology
Fluid Flow and Transfer Processes

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10.1021/acssensors.1c01101

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title = "Nanoplasmonic Sandwich Immunoassay for Tumor-Derived Exosome Detection and Exosomal PD-L1 Profiling",

abstract = "Tumor-derived exosomes play a vital role in the process of cancer development. Quantitative analysis of exosomes and exosome-shuttled proteins would be of immense value in understanding cancer progression and generating reliable predictive biomarkers for cancer diagnosis and treatment. Recent studies have indicated the critical role of exosomal programmed death ligand 1 (PD-L1) in immune checkpoint therapy and its application as a patient stratification biomarker in cancer immunotherapy. Here, we present a nanoplasmonic exosome immunoassay utilizing gold-silver (Au@Ag) core-shell nanobipyramids and gold nanorods, which form sandwich immune complexes with target exosomes. The immunoassay generates a distinct plasmonic signal pattern unique to exosomes with specific exosomal PD-L1 expression, allowing rapid, highly sensitive exosome detection and accurate identification of PD-L1 exosome subtypes in a single assay. The developed nanoplasmonic sandwich immunoassay provides a novel and viable approach for tumor cell-derived exosome detection and analysis with quantitative molecular details of key exosomal proteins, manifesting its great potential as a transformative diagnostic tool for early cancer detection, prognosis, and post-treatment monitoring.",

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author = "Chuanyu Wang and Huang, {Chung Hui} and Zhuangqiang Gao and Jialiang Shen and Jiacheng He and Alana MacLachlan and Chao Ma and Ya Chang and Wen Yang and Yuxin Cai and Yang Lou and Siyuan Dai and Weiqiang Chen and Feng Li and Pengyu Chen",

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T1 - Nanoplasmonic Sandwich Immunoassay for Tumor-Derived Exosome Detection and Exosomal PD-L1 Profiling

AU - Wang, Chuanyu

AU - Huang, Chung Hui

AU - Gao, Zhuangqiang

AU - Shen, Jialiang

AU - He, Jiacheng

AU - MacLachlan, Alana

AU - Ma, Chao

AU - Chang, Ya

AU - Yang, Wen

AU - Cai, Yuxin

AU - Lou, Yang

AU - Dai, Siyuan

AU - Chen, Weiqiang

AU - Li, Feng

AU - Chen, Pengyu

PY - 2021/9/24

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N2 - Tumor-derived exosomes play a vital role in the process of cancer development. Quantitative analysis of exosomes and exosome-shuttled proteins would be of immense value in understanding cancer progression and generating reliable predictive biomarkers for cancer diagnosis and treatment. Recent studies have indicated the critical role of exosomal programmed death ligand 1 (PD-L1) in immune checkpoint therapy and its application as a patient stratification biomarker in cancer immunotherapy. Here, we present a nanoplasmonic exosome immunoassay utilizing gold-silver (Au@Ag) core-shell nanobipyramids and gold nanorods, which form sandwich immune complexes with target exosomes. The immunoassay generates a distinct plasmonic signal pattern unique to exosomes with specific exosomal PD-L1 expression, allowing rapid, highly sensitive exosome detection and accurate identification of PD-L1 exosome subtypes in a single assay. The developed nanoplasmonic sandwich immunoassay provides a novel and viable approach for tumor cell-derived exosome detection and analysis with quantitative molecular details of key exosomal proteins, manifesting its great potential as a transformative diagnostic tool for early cancer detection, prognosis, and post-treatment monitoring.

AB - Tumor-derived exosomes play a vital role in the process of cancer development. Quantitative analysis of exosomes and exosome-shuttled proteins would be of immense value in understanding cancer progression and generating reliable predictive biomarkers for cancer diagnosis and treatment. Recent studies have indicated the critical role of exosomal programmed death ligand 1 (PD-L1) in immune checkpoint therapy and its application as a patient stratification biomarker in cancer immunotherapy. Here, we present a nanoplasmonic exosome immunoassay utilizing gold-silver (Au@Ag) core-shell nanobipyramids and gold nanorods, which form sandwich immune complexes with target exosomes. The immunoassay generates a distinct plasmonic signal pattern unique to exosomes with specific exosomal PD-L1 expression, allowing rapid, highly sensitive exosome detection and accurate identification of PD-L1 exosome subtypes in a single assay. The developed nanoplasmonic sandwich immunoassay provides a novel and viable approach for tumor cell-derived exosome detection and analysis with quantitative molecular details of key exosomal proteins, manifesting its great potential as a transformative diagnostic tool for early cancer detection, prognosis, and post-treatment monitoring.

KW - exosomal protein

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KW - gold−silver core−shell nanobipyramids (Au@Ag NBPs)

KW - immunoassay

KW - localized surface plasmon resonance (LSPR)

KW - microfluidics

KW - programmed death ligand 1 (PD-L1)

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Nanoplasmonic Sandwich Immunoassay for Tumor-Derived Exosome Detection and Exosomal PD-L1 Profiling

Abstract

Keywords

ASJC Scopus subject areas

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