TY - JOUR
T1 - Nanoscale-localized multiplexed biological activation of field effect transistors for biosensing applications
AU - Wright, Alexander James
AU - Nasralla, Hashem Hassan
AU - Deshmukh, Rahul
AU - Jamalzadeh, Moeid
AU - Hannigan, Matthew
AU - Patera, Andrew
AU - Li, Yanxiao
AU - Manzo-Perez, Miguel
AU - Parashar, Nitika
AU - Huang, Zhujun
AU - Udumulla, Thanuka
AU - Chen, Weiqiang
AU - De Forni, Davide
AU - Weck, Marcus
AU - de Peppo, Giuseppe Maria
AU - Riedo, Elisa
AU - Shahrjerdi, Davood
N1 - Publisher Copyright:
© 2024 The Royal Society of Chemistry.
PY - 2024/9/24
Y1 - 2024/9/24
N2 - The rise in antibiotic-resistant pathogens, highly infectious viruses, and chronic diseases has prompted the search for rapid and versatile medical tests that can be performed by the patient. Field-effect transistor (FET)-based electronic biosensing platforms are particularly attractive due to their sensitivity, fast turn-around time, potential for parallel detection of multiple pathogens, and compatibility with semiconductor manufacturing. However, an unmet critical need is a scalable, site-selective multiplexed biofunctionalization method with nanoscale precision for immobilizing different types of pathogen-specific bioreceptors on individual FETs, preventing parallel detection of multiple targets. Here, we propose a paradigm shift in FET biofunctionalization using thermal scanning probe lithography (tSPL) with a thermochemically sensitive polymer. This polymer can be spin-coated on fully-fabricated FET chips, making this approach applicable to any FET sensor material and technology. Crucially, we demonstrate the spatially selective multiplexed functionalization capability of this method by immobilizing different types of bioreceptors at prescribed locations on a chip with sub-20 nm resolution, paving the way for massively parallel FET detection of multiple pathogens. Antibody- and aptamer-modified graphene FET sensors are then realized, achieving ultra-sensitive detection of a minimum measured concentrations of 3 aM of SARS-CoV-2 spike proteins and 10 human SARS-CoV-2 infectious live virus particles per ml, and selectivity against human influenza A (H1N1) live virus.
AB - The rise in antibiotic-resistant pathogens, highly infectious viruses, and chronic diseases has prompted the search for rapid and versatile medical tests that can be performed by the patient. Field-effect transistor (FET)-based electronic biosensing platforms are particularly attractive due to their sensitivity, fast turn-around time, potential for parallel detection of multiple pathogens, and compatibility with semiconductor manufacturing. However, an unmet critical need is a scalable, site-selective multiplexed biofunctionalization method with nanoscale precision for immobilizing different types of pathogen-specific bioreceptors on individual FETs, preventing parallel detection of multiple targets. Here, we propose a paradigm shift in FET biofunctionalization using thermal scanning probe lithography (tSPL) with a thermochemically sensitive polymer. This polymer can be spin-coated on fully-fabricated FET chips, making this approach applicable to any FET sensor material and technology. Crucially, we demonstrate the spatially selective multiplexed functionalization capability of this method by immobilizing different types of bioreceptors at prescribed locations on a chip with sub-20 nm resolution, paving the way for massively parallel FET detection of multiple pathogens. Antibody- and aptamer-modified graphene FET sensors are then realized, achieving ultra-sensitive detection of a minimum measured concentrations of 3 aM of SARS-CoV-2 spike proteins and 10 human SARS-CoV-2 infectious live virus particles per ml, and selectivity against human influenza A (H1N1) live virus.
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U2 - 10.1039/d4nr02535k
DO - 10.1039/d4nr02535k
M3 - Article
C2 - 39324869
AN - SCOPUS:85205436894
SN - 2040-3364
VL - 16
SP - 19620
EP - 19632
JO - Nanoscale
JF - Nanoscale
IS - 42
ER -