TY - JOUR
T1 - Nanotopographical surfaces for stem cell fate control
T2 - Engineering mechanobiology from the bottom
AU - Chen, Weiqiang
AU - Shao, Yue
AU - Li, Xiang
AU - Zhao, Gang
AU - Fu, Jianping
N1 - Funding Information:
We acknowledge financial support from the National Science Foundation (CMMI 1129611 and CBET 1149401), the National Institutes of Health ( R21 HL114011 and R21 EB017078 ), the American Heart Association ( 12SDG12180025 ), the UM Comprehensive Cancer Center Prostate SPORE Pilot Project ( P50 CA069568 ), and the Michigan Institute for Clinical & Health Research (MICHR) Pilot Program ( UL1 RR024986 ). W. Chen is supported in part by the American Heart Association Predoctoral Fellowship ( 13PRE16510018 ). Finally, we extend our apologies to all our colleagues in the field whose work we are unable to discuss formally because of space constraints.
PY - 2014/12/1
Y1 - 2014/12/1
N2 - During embryogenesis and tissue maintenance and repair in an adult organism, a myriad of stem cells are regulated by their surrounding extracellular matrix (ECM) enriched with tissue/organ-specific nanoscale topographical cues to adopt different fates and functions. Attributed to their capability of self-renewal and differentiation into most types of somatic cells, stem cells also hold tremendous promise for regenerative medicine and drug screening. However, a major challenge remains as to achieve fate control of stem cells in vitro with high specificity and yield. Recent exciting advances in nanotechnology and materials science have enabled versatile, robust, and large-scale stem cell engineering in vitro through developments of synthetic nanotopographical surfaces mimicking topological features of stem cell niches. In addition to generating new insights for stem cell biology and embryonic development, this effort opens up unlimited opportunities for innovations in stem cell-based applications. This review is therefore to provide a summary of recent progress along this research direction, with perspectives focusing on emerging methods for generating nanotopographical surfaces and their applications in stem cell research. Furthermore, we provide a review of classical as well as emerging cellular mechano-sensing and -transduction mechanisms underlying stem cell nanotopography sensitivity and also give some hypotheses in regard to how a multitude of signaling events in cellular mechanotransduction may converge and be integrated into core pathways controlling stem cell fate in response to extracellular nanotopography.
AB - During embryogenesis and tissue maintenance and repair in an adult organism, a myriad of stem cells are regulated by their surrounding extracellular matrix (ECM) enriched with tissue/organ-specific nanoscale topographical cues to adopt different fates and functions. Attributed to their capability of self-renewal and differentiation into most types of somatic cells, stem cells also hold tremendous promise for regenerative medicine and drug screening. However, a major challenge remains as to achieve fate control of stem cells in vitro with high specificity and yield. Recent exciting advances in nanotechnology and materials science have enabled versatile, robust, and large-scale stem cell engineering in vitro through developments of synthetic nanotopographical surfaces mimicking topological features of stem cell niches. In addition to generating new insights for stem cell biology and embryonic development, this effort opens up unlimited opportunities for innovations in stem cell-based applications. This review is therefore to provide a summary of recent progress along this research direction, with perspectives focusing on emerging methods for generating nanotopographical surfaces and their applications in stem cell research. Furthermore, we provide a review of classical as well as emerging cellular mechano-sensing and -transduction mechanisms underlying stem cell nanotopography sensitivity and also give some hypotheses in regard to how a multitude of signaling events in cellular mechanotransduction may converge and be integrated into core pathways controlling stem cell fate in response to extracellular nanotopography.
KW - Biomaterials
KW - Mechanobiology
KW - Nanotopography
KW - Regenerative medicine
KW - Stem cell
KW - Tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=84935018276&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84935018276&partnerID=8YFLogxK
U2 - 10.1016/j.nantod.2014.12.002
DO - 10.1016/j.nantod.2014.12.002
M3 - Review article
AN - SCOPUS:84935018276
VL - 9
SP - 759
EP - 784
JO - Nano Today
JF - Nano Today
SN - 1748-0132
IS - 6
ER -