Nanotopography influences adhesion, spreading, and self-renewal of Human embryonic stem cells

Weiqiang Chen, Luis G. Villa-Diaz, Yubing Sun, Shinuo Weng, Jin Koo Kim, Raymond H.W. Lam, Lin Han, Rong Fan, Paul H. Krebsbach, Jianping Fu

Research output: Contribution to journalArticlepeer-review


Human embryonic stem cells (hESCs) have great potentials for future cell-based therapeutics. However, their mechanosensitivity to biophysical signals from the cellular microenvironment is not well characterized. Here we introduced an effective microfabrication strategy for accurate control and patterning of nanoroughness on glass surfaces. Our results demonstrated that nanotopography could provide a potent regulatory signal over different hESC behaviors, including cell morphology, adhesion, proliferation, clonal expansion, and self-renewal. Our results indicated that topological sensing of hESCs might include feedback regulation involving mechanosensory integrin-mediated cell-matrix adhesion, myosin II, and E-cadherin. Our results also demonstrated that cellular responses to nanotopography were cell-type specific, and as such, we could generate a spatially segregated coculture system for hESCs and NIH/3T3 fibroblasts using patterned nanorough glass surfaces.

Original languageEnglish (US)
Pages (from-to)4094-4103
Number of pages10
JournalACS nano
Issue number5
StatePublished - May 22 2012


  • Human embryonic stem cell
  • Mechanosensitivity
  • Microfabrication
  • Nanotopography
  • Self-renewal

ASJC Scopus subject areas

  • General Materials Science
  • General Engineering
  • General Physics and Astronomy


Dive into the research topics of 'Nanotopography influences adhesion, spreading, and self-renewal of Human embryonic stem cells'. Together they form a unique fingerprint.

Cite this