Near quantitative production of molecular nitrogen from metabolism of dimethylnitrosamine

S. Milstein; J. B. Guttenplan

doi:10.1016/0006-291X(79)91684-X

Near quantitative production of molecular nitrogen from metabolism of dimethylnitrosamine

S. Milstein, J. B. Guttenplan

Molecular Pathobiology

Research output: Contribution to journal › Article › peer-review

Abstract

Molecular nitrogen is produced stoichiometrically from the spontaneous decomposition of N-methyl-N-nitrosourea in phosphate buffer and is readily detected by gas chromatography. It is also generated in high yield from the oxidation of dimethylnitrosamine by mouse or rat liver microsomes. Gas chromatographic analysis for nitrogen generated from dimethylnitrosamine was more difficult because the amount of nitrogen generated was similar to that produced in control tubes which contained no dimethylnitrosamine.

Original language	English (US)
Pages (from-to)	337-342
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	87
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(79)91684-X
State	Published - Mar 15 1979

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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AB - Molecular nitrogen is produced stoichiometrically from the spontaneous decomposition of N-methyl-N-nitrosourea in phosphate buffer and is readily detected by gas chromatography. It is also generated in high yield from the oxidation of dimethylnitrosamine by mouse or rat liver microsomes. Gas chromatographic analysis for nitrogen generated from dimethylnitrosamine was more difficult because the amount of nitrogen generated was similar to that produced in control tubes which contained no dimethylnitrosamine.

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Near quantitative production of molecular nitrogen from metabolism of dimethylnitrosamine

Abstract

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