Molecular nitrogen is produced stoichiometrically from the spontaneous decomposition of N-methyl-N-nitrosourea in phosphate buffer and is readily detected by gas chromatography. It is also generated in high yield from the oxidation of dimethylnitrosamine by mouse or rat liver microsomes. Gas chromatographic analysis for nitrogen generated from dimethylnitrosamine was more difficult because the amount of nitrogen generated was similar to that produced in control tubes which contained no dimethylnitrosamine.
|Original language||English (US)|
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Mar 15 1979|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology