Neural form of voltage-dependent sodium current in human cultured dental pulp cells

Research output: Contribution to journalArticle

Abstract

Intradental, i.e. pulpal, cells may play an important part in sensory transduction in teeth, although the cellular mechanisms and the identity of the specific cell types involved are still unclear. Because the majority of cells in dental pulp are derived from neural crest, it seemed likely that these might have the membrane properties of other neural-derived cells found in the peripheral or central nervous system. The patch-clamp recording technique was used to show that cells in explant cultures from human dental pulp contain a voltage-gated, tetrodotoxin-sensitive inward current. Mean activation potential of the current was -42 ± 2.5 mV and the voltage at half-inactivation was -79.4 ± 5.3 mV, suggesting a neural-like sodium conductance. In addition, these cells were immunoreactive to glial acidic fibrillary protein, growth-associated protein (GAP-43), and vimentin, further suggesting that dental pulp contains a population of cells with membrane properties similar to neuronal satellite cells. These cells may contribute, either directly or indirectly, to somatosensation in teeth.

Original languageEnglish (US)
Pages (from-to)613-620
Number of pages8
JournalArchives of Oral Biology
Volume39
Issue number7
DOIs
StatePublished - Jul 1994

Keywords

  • GAP-43
  • glial fibrillary acidic protein
  • immunocytochemistry
  • odontoblast
  • patch-clamping
  • vimentin

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

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