Neutrophil dynamics during migration in microfluidic concentration gradients

Mohammad A. Qasaimeh, Melina Astolfi, Michal Pyzik, Silvia Vidal, David Juncker

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports on the study of neutrophil dynamics during chemotaxis using the Microfluidic Quadrupole (MQ). The MQ is used to generate concentration gradients of the chemokine Interleukin-8 atop human neutrophils cultured in petri-dishes. The gradients are generated within the MQ in the gap between the microfluidic probe and the neutrophil-containing dish. We studied travelled distances and migration speeds of individual neutrophils using real time-lapse microscopy and observed heterogeneity with neutrophils dynamics. Results show that the migration distance of individual neutrophils is correlated to their initial position within the gradients. Interestingly, analysis revealed that neutrophils started migration with full speed at the beginning of the experiment when exposed to the gradients, and then slow down as they migrate toward higher concentrations until they stop, suggesting a desensitization mechanism that is gradually activated.

Original languageEnglish (US)
Title of host publicationProceedings - 2014 40th Annual Northeast Bioengineering Conference, NEBEC 2014
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781479937288
DOIs
StatePublished - Dec 2 2014
Event2014 40th Annual Northeast Bioengineering Conference, NEBEC 2014 - Boston, United States
Duration: Apr 25 2014Apr 27 2014

Publication series

NameProceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC
Volume2014-December
ISSN (Print)1071-121X
ISSN (Electronic)2160-7001

Other

Other2014 40th Annual Northeast Bioengineering Conference, NEBEC 2014
CountryUnited States
CityBoston
Period4/25/144/27/14

Keywords

  • Chemotaxis
  • Concentration gradients
  • Microfluidics
  • Migration dynamics
  • Neutrophils

ASJC Scopus subject areas

  • Bioengineering

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  • Cite this

    Qasaimeh, M. A., Astolfi, M., Pyzik, M., Vidal, S., & Juncker, D. (2014). Neutrophil dynamics during migration in microfluidic concentration gradients. In Proceedings - 2014 40th Annual Northeast Bioengineering Conference, NEBEC 2014 [6972913] (Proceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC; Vol. 2014-December). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NEBEC.2014.6972913