TY - GEN
T1 - Neutrophil dynamics during migration in microfluidic concentration gradients
AU - Qasaimeh, Mohammad A.
AU - Astolfi, Melina
AU - Pyzik, Michal
AU - Vidal, Silvia
AU - Juncker, David
N1 - Publisher Copyright:
© 2014 IEEE.
PY - 2014/12/2
Y1 - 2014/12/2
N2 - This paper reports on the study of neutrophil dynamics during chemotaxis using the Microfluidic Quadrupole (MQ). The MQ is used to generate concentration gradients of the chemokine Interleukin-8 atop human neutrophils cultured in petri-dishes. The gradients are generated within the MQ in the gap between the microfluidic probe and the neutrophil-containing dish. We studied travelled distances and migration speeds of individual neutrophils using real time-lapse microscopy and observed heterogeneity with neutrophils dynamics. Results show that the migration distance of individual neutrophils is correlated to their initial position within the gradients. Interestingly, analysis revealed that neutrophils started migration with full speed at the beginning of the experiment when exposed to the gradients, and then slow down as they migrate toward higher concentrations until they stop, suggesting a desensitization mechanism that is gradually activated.
AB - This paper reports on the study of neutrophil dynamics during chemotaxis using the Microfluidic Quadrupole (MQ). The MQ is used to generate concentration gradients of the chemokine Interleukin-8 atop human neutrophils cultured in petri-dishes. The gradients are generated within the MQ in the gap between the microfluidic probe and the neutrophil-containing dish. We studied travelled distances and migration speeds of individual neutrophils using real time-lapse microscopy and observed heterogeneity with neutrophils dynamics. Results show that the migration distance of individual neutrophils is correlated to their initial position within the gradients. Interestingly, analysis revealed that neutrophils started migration with full speed at the beginning of the experiment when exposed to the gradients, and then slow down as they migrate toward higher concentrations until they stop, suggesting a desensitization mechanism that is gradually activated.
KW - Chemotaxis
KW - Concentration gradients
KW - Microfluidics
KW - Migration dynamics
KW - Neutrophils
UR - http://www.scopus.com/inward/record.url?scp=84940688540&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84940688540&partnerID=8YFLogxK
U2 - 10.1109/NEBEC.2014.6972913
DO - 10.1109/NEBEC.2014.6972913
M3 - Conference contribution
AN - SCOPUS:84940688540
T3 - Proceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC
BT - Proceedings - 2014 40th Annual Northeast Bioengineering Conference, NEBEC 2014
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2014 40th Annual Northeast Bioengineering Conference, NEBEC 2014
Y2 - 25 April 2014 through 27 April 2014
ER -