Neutrophils migrate longer distances in moving microfluidic concentration gradients compared to static ones

Mohammad A. Qasaimeh, Mélina Astolfi, Michal Pyzik, Silvia Vidal, David Juncker

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present the use of our recently developed microfluidic quadrupole (MQ) to generate controllable microfluidic floating concentration gradients of 25 ng·ml-1 Interleukin 8 (IL-8), and challenge neutrophils cultured in petri-dishes with stationary and moving gradients. The dynamic responses of individual neutrophils were analyzed and we found that the migration length of neutrophils is correlated to their initial position within the stationary gradient. However, for moving gradients this correlation vanishes, while at the same time neutrophils migrate over longer distances. Our results and demonstrated experimentation lay the foundation for future novel chemotaxis assays under moving gradients of different shapes and speeds.

Original languageEnglish (US)
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages2007-2009
Number of pages3
ISBN (Print)9781632666246
StatePublished - 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume3

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Country/TerritoryGermany
CityFreiburg
Period10/27/1310/31/13

Keywords

  • Chemotaxis
  • Floating gradients
  • Microfluidic probe
  • Microfluidic quadrupole
  • Neutrophils

ASJC Scopus subject areas

  • Bioengineering

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