@article{bafbad83a57d440283e1e61ee03940d1,
title = "Next-generation sequencing to generate interactome datasets",
abstract = "Next-generation sequencing has not been applied to protein-protein interactome network mapping so far because the association between the members of each interacting pair would not be maintained in en masse sequencing. We describe a massively parallel interactome-mapping pipeline, Stitch-seq, that combines PCR stitching with next-generation sequencing and used it to generate a new human interactome dataset. Stitch-seq is applicable to various interaction assays and should help expand interactome network mapping.",
author = "Haiyuan Yu and Leah Tardivo and Stanley Tam and Evan Weiner and Fana Gebreab and Changyu Fan and Nenad Svrzikapa and Tomoko Hirozane-Kishikawa and Edward Rietman and Xinping Yang and Julie Sahalie and Kourosh Salehi-Ashtiani and Tong Hao and Cusick, {Michael E.} and Hill, {David E.} and Roth, {Frederick P.} and Pascal Braun and Marc Vidal",
note = "Funding Information: We thank members of the Dana-Farber Cancer Institute Center for Cancer Systems Biology and the Roth Laboratory for helpful discussions, and members of the University of Pennsylvania DNA Sequencing Facility in the Department of Genetics for the 454 FLX sequencing. This work was funded in part by grants R01 HG001715 (to D.E.H., F.P.R. and M.V.) and R21 HG004756 (to F.P.R.) from the US National Human Genome Research Institute of the National Institutes of Health, a grant from the Ellison Foundation (to M.V.), a Canadian Institute for Advanced Research Fellowship and Canada Excellence Research Chair (to F.P.R.) and in part by Institute Sponsored Research funds from the Dana-Farber Cancer Institute Strategic Initiative in support of Center for Cancer Systems Biology. M.V. is supported as a Chercheur Qualifi{\'e} Honoraire from the Fonds de la Recherche Scientifique (French Community of Belgium).",
year = "2011",
month = jun,
doi = "10.1038/nmeth.1597",
language = "English (US)",
volume = "8",
pages = "478--480",
journal = "Nature methods",
issn = "1548-7091",
publisher = "Nature Research",
number = "6",
}