Nitrate reductase-mediated synthesis of silver nanoparticles from AgNO 3

S. Anil Kumar, Majid Kazemian Abyaneh, S. W. Gosavi, Sulabha K. Kulkarni, Renu Pasricha, Absar Ahmad, M. I. Khan

Research output: Contribution to journalArticlepeer-review


Synthesis of silver nanoparticles using α-NADPH-dependent nitrate reductase and phytochelatin in vitro has been demonstrated for the first time. The silver ions were reduced in the presence of nitrate reductase, leading to the formation of a stable silver hydrosol 10-25 nm diam. and stabilized by the capping peptide. The nanoparticles were characterized by X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy and UV-Vis absorption. These studies will help in designing a rational enzymatic strategy for the synthesis of nanomaterials of different chemical composition, shapes and sizes as well as their separation.

Original languageEnglish (US)
Pages (from-to)439-445
Number of pages7
JournalBiotechnology Letters
Issue number3
StatePublished - Mar 2007


  • Capping peptide
  • Enzymatic method
  • Nitrate reductase
  • Silver nanoparticles

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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